Yi Sheng Wang, Ph.D.
Name Yi Sheng Wang, Ph.D.
Incumbent Professor
所屬單位 Deputy Director
Acting Division Director of Physical & Computational Genomics


Ph.D., Chemistry, National Taiwan University, 2001
NCLscan:
研究方向/領域

RESEARCH INTERESTS

Biophysical Chemistry and Technology Development

Our objective is the advancement of bio-analytical sciences and biophysics in a systematic and complementary manner. Both fundamental biophysical knowledge and technical innovations are essential for resolving critical problems, such as high-throughput diagnoses, accurate quantifications and molecular function examinations. We have studied the physics and chemistries involved in the development of key analytical technologies, and integrated them for express clinical diagnoses. The fundamental study of ionization mechanism of biomolecules is one of our focuses, which enables us to design an effective strategy to improve the detection of carbohydrates. The technologies developed in this laboratory include:

  • Synchronized Dual-Polarity Mass Spectrometry
  • Synchronized Dual-Polarity Imaging Mass Spectrometry
  • Soft Protonation Method for Carbohydrates
  • Ultra-Sensitive Surface Ionization Method for Carbohydrates
  • Structural and Energetic Simulation of Oligosaccharides
  • Nanoparticles for Selective Purification of Phosphoproteins

Dual-polarity imaging_mass
Dual-polarity imaging mass spectrometer and analytical results of insulin A-chain with matrix on surface. 
EDUCATION AND POSITIONS HELD
  • 2025-present Acting Division director of the Physical and Computational Genomics division, Genomics Research Center, Academia Sinica
  • 2023-present Deputy Director, Genomics Research Center, Academia Sinica
  • 2019-present Professor, Genomics Research Center, Academia Sinica
  • 2011-2019 Associate Professor, Genomics Research Center, Academia Sinica
  • 2005-2011 Assistant Professor, Genomics Research Center, Academia Sinica
  • 2002-2003 Postdoctoral Fellow, National High Magnetic Field Laboratory (NHMFL), Florida State University, U.S.A.
  • 2001-2005 Postdoctoral Fellow, Institute of Atomic and Molecular Sciences (IAMS), Academia Sinica
HONORS
  • 2004 Outstanding Post-Doctor Researchers Conference Travel Grant
  • 2002-2003 Advanced Abroad Research Fellowship
  • 2002 Outstanding Post-Doctor Researchers Conference Travel Grant
  • 2000 Outstanding Students Conference Travel Grant
Year Paper Title
2026 Yi-Hong Cai, Yi-Sheng Wang*, Theoretical Study of High-Order Velocity Focusing Achieved with Single-Stage Reflectron Time-of-flight Mass Spectrometry, Journal of the American Society for Mass Spectrometry, vol. 37 (1), 33-41, 2026具代表性; 任職中研院著作;
2025 Cao Jie-Ling, Guo Jie-He, Leung Will, Liu Ya-Ting, Tsai I-Lin, Huang Shu-Ling, (Wang Yi-Sheng)*, Lai Yin-Hung*, Do phenolic contents relate to their in-vitro cytotoxicity against colorectal cancer cell lines from Calvatia lilacina (puffball mushroom)?, Food Bioscience, vol. 106193, 2025任職中研院著作;
2025 Ali Farhan, Yi-Sheng Wang*, Recent applications of artificial intelligence and related technical challenges in MALDI MS and MALDI-MSI – A mini review, Mass Spectrometry (MSSJ), vol. 14 (1), A0175-A0175, A0175-A0175, 2025任職中研院著作;
2025 Liang Yu-Jen, Yang Chih-Ting, Chen Chia-Wei, Lin Yin-Chun, Lin Shu-Yao, Wang Yi Sheng, Yang Hsin-Chou*, SMART 2.0 Statistical Metabolomics Analysis: An R Tool 2.0, Analytical Chemistry, vol. 97 (46), 25453-25468, 2025任職中研院著作;
2024 Cai YH, Wang CC, Hsiao CH, (Wang YS)*, Experimental Validation of Comprehensive Calculation for High-Resolution Linear MALDI-TOF Mass Spectrometry., Journal of the American Society for Mass Spectrometry, vol. 35(5), 992-998, 2024任職中研院著作;
2024 Chang Ko‐Keng, Ko Yi‐Yu, Chou Po‐Yu, Lai Szu‐Hsueh, (Wang Yi‐Sheng)*, MAXTOF: An efficient calculation tool for precise optimization of MALDI-TOF MS measurements in real time‐TOF MS measurements in real time, Journal of the Chinese Chemical Society, vol. 71(8) 932-941, 2024任職中研院著作;
2023 Lai YH*, Leung W, Chang PH, Zhou WX, (Wang YS*, Structural identification of carbohydrate isomers using ambient infrared-assisted dissociation., Analytica chimica acta, vol. 1264, 341307, 2023任職中研院著作;
2023 Lai Yin‐Hung*, (Wang Yi‐Sheng)*, Advances in high‐resolution mass spectrometry techniques for analysis of high mass‐to‐charge ions, Mass Spectrometry Reviews, vol. 42(6):2426-2445, 2023具代表性; 任職中研院著作;
2023 Yi-Hong Cai, Cheng-Huang Lin, Yi-Sheng Wang, Theoretical study of general principle of high-resolution MALDI-linear time-of-flight mass spectrometry in middle to high m/z ranges, INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, vol. 489, 117052, 117052, 2023具代表性; 任職中研院著作;
2022 Chi CH, (Wang YS, Ng LT*, Structural characterization of mushroom polysaccharides by cyclic ion mobility-mass spectrometry., Journal of chromatography. A, vol. 1680, 463445, 2022任職中研院著作;
2022 Chang Ko-Keng, Cai Yi-Hong, Hsiao Chih-Hao, Hsu Cheng-Chih, (Wang Yi-Sheng)*, High-performance miniature linear time-of-flight mass spectrometry as an advantageous tool in a high mass-to-charge range, The Analyst, vol. 147(18), 4116-4123, 2022具代表性; 任職中研院著作;
2022 Cai Yi-Hong, Lin Cheng-Huang, (Wang Yi-Sheng)*, Theoretical study of the impact of ion acceleration parameters on the mass resolving power in linear MALDI time-of-flight mass spectrometry, International Journal of Mass Spectrometry, vol. 471, 116756, 2022任職中研院著作;
2022 Yong Tracer, Chang Ko-Keng, (Wang Yi-Sheng, Ma Che*, Active Humoral Response Reverts Tumorigenicity through Disruption of Key Signaling Pathway, Vaccines, vol. 10(2), 163, 2022任職中研院著作;
2020 C.-H. Hsiao, Y.-H. Lai, S.-Y. Kuo, Y.-H. Cai, C.-H. Lin, (Y.-S. Wang)*, A Dynamic Data Correction Method for Enhancing Resolving Power of Integrated Spectra in Spectroscopic Analysis, ANALYTICAL CHEMISTRY, vol. 92(19), 12763-12768, 2020具代表性; 任職中研院著作;
2019 Wang Chia-Chen, (Wang Yi-Sheng)*, Effective analysis of degree of polymerization of polysialic acids in mass spectrometry by combining novel sample preparation and dynamic instrument optimization methods, Carbohydrate Research, vol. 471, 78-84, 2019具代表性; 任職中研院著作;
2018 Cai Yi-Hong, Wang Yi-Sheng*, Impact of uneven sample morphology on mass resolving power in linear MALDI-TOF mass spectrometry: A comprehensive theoretical investigation, Journal of Mass Spectrometry, vol. 53(4), 361-368, 2018具代表性; 任職中研院著作;
2018 Wei Shih-Chun, Fan Shen, Lien Chia-Wen, Unnikrishnan Binesh, Wang Yi-Sheng, Chu Han-Wei, Huang Chih-Ching, Hsu Pang-Hung, Chang Huan-Tsung*, Graphene oxide membrane as an efficient extraction and ionization substrate for spray-mass spectrometric analysis of malachite green and its metabolite in fish samples, Analytica Chimica Acta, vol. 1003, 42-48, 2018任職中研院著作;
2018 Ou Yu-Meng, Kuo Shu-Yun, Lee Hsun, Chang Huan-Tsung, Wang Yi-Sheng*, An Efficient Sample Preparation Method to Enhance Carbohydrate Ion Signals in Matrix-assisted Laser Desorption/Ionization Mass Spectrometry, Jove-Journal of Visualized Experiments, vol. 137, e57660, 2018具代表性; 任職中研院著作;
2017 H. Lee, Y.-H. Lai, Y.-M. Ou, C.-W. Tsao, Y.-J. Jheng, S.-Y. Kuo, H.-T. Chang, Y.-S. Wang*, Enhancing carbohydrate ion yield by controlling crystalline structures in matrix-assisted laser desorption/ionization mass spectrometry, ANALYTICA CHIMICA ACTA, vol. 994, 49-55, 2017具代表性; 任職中研院著作;
2016 Y.-H. Lai, Y.-H. Cai, H. Lee, Y.-M. Ou, C.-H. Hsiao, C.-W. Tsao, H.-T. Chang, Y.-S. Wang*, Reducing spatial heterogeneity of MALDI samples with Marangoni flows during sample preparation, JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, vol. 27(8), 1314-1321, 2016具代表性; 任職中研院著作;
2016 Chang, K.-K.; Cai, Y.-H.; Chen, C.-H.; Wang, Y.-S.;*, Neutralization charge detection method for detecting ions under ambient and liquid phases., CHEMICAL COMMUNICATIONS, vol. 52(29), 5187-5189, 2016具代表性; 任職中研院著作;
2016 Dutkiewicz E. P., Hsieh K.-T., Wang Y.-S., Chiu H.-Y., Urban P. L., Hydrogel Micropatch and Mass Spectrometry-Assisted Screening for Psoriasis-Related Skin Metabolites, Clinical Chemistry, vol. 62(8), 1120-1128, 2016任職中研院著作;
2016 Primadona Indah, Lai Yin-Hung, Capangpangan Rey Y., Obena Rofeamor P., Tseng Mei-Chun, Huang Ming-Feng, Chang Huan-Tsung, Li Shiou-Ting, Wu Chung-Yi, Chien Wei-Ting, Lin Chun-Cheng, Wang Yi-Sheng, * Chen Yu-Ju*, Functionalized HgTe nanoparticles promote laser-induced solid phase ionization/dissociation for comprehensive glycan sequencing, The Analyst, vol. 141(21), 6093-6103, 2016任職中研院著作;
2016 Y.-M. Ou, C.-W. Tsao, Y.-H. Lai, H. Lee, H.-T. Chang, Y.-S. Wang*, Preparation of homogeneous MALDI samples for quantitative applications, JOVE-Journal of Visualized experiments, vol. 116, e54409, 2016具代表性; 任職中研院著作;
2016 C.-C. Wang, Y.-H. Lai, Y.-M. Ou, H.-T. Chang, Y.-S. Wang*, Critical Factors Determining Quantification Capability of MALDI-TOF Mass Spectrometry, PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY A-MATHEMATICAL PHYSICAL AND ENGINEERING SCIENCES, vol. 374(2079), 20150371, 2016具代表性; 任職中研院著作;
2015 Cai, Y.-H.; Lai, Y.-H.; Wang, Y.-S.*, Coupled space- and velocity-focusing in time-of-flight mass spectrometry – a comprehensive theoretical investigation., JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, vol. 26(10), 1722-1731, 2015具代表性; 任職中研院著作;
2014 Dutkiewicz, E.; Lin, J.-D.; Tseng, T.-W.; Wang, Y.-S.; Urban, P.*, Hydrogel micropatches for sampling and profiling skin metabolome., ANALYTICAL CHEMISTRY, vol. 86 (5), 2337-2344, 2014任職中研院著作;
2014 Lai, Y.-H.; Chen, B.-G.; Lee, Y. T.; Wang, Y.-S.;* Lin, S. H.*, Contribution of thermal energy to initial ion production of matrix-assisted laser desorption/ionization observed with 2,4,6-trihydroxyacetophenone., RAPID COMMUNICATIONS IN MASS SPECTROMETRY, vol. 1716-1722, 2014具代表性; 任職中研院著作;
2014 Ozdemir, A.; Lin, J.-L.; Wang, Y.-S.; Chen, C.-H.*, A deeper look into sonic spray ionization., RSC ADVANCES, vol. 4(106), 61290-61297, 2014任職中研院著作;
2013 C.-L. Wu, C.-C. Wang, Y.-H. Lai, H. Lee, J.-D. Lin, Y. T. Lee, (Y.-S. Wang)*, Selective enhancement of carbohydrate ion abundances by diamond nanoparticles for mass spectrometric analysis., ANALYTICAL CHEMISTRY, vol. 85, 3836-3841, 2013具代表性; 任職中研院著作;
2012 O. P. Charkin, * N. M. Klimenko, (Y.-S. Wang, Theoretical Study of C60(OH)20 and C60(OH)18 fullerenols and B12(OH)122-, B12(OH)122-, and B12(OH)122- polyhydroxyl clusters and their Li-substituted derivatives., RUSSIAN JOURNAL OF INORGANIC CHEMISTRY, vol. 57(7), 970-980, 2012任職中研院著作;
2012 Y.-H. Lai, C.-C. Wang, B.-H. Liu, C. W. Chen, S.-H. Lin, Y. T. Lee, (Y.-S. Wang)*, Analysis of initial reactions of MALDI based on chemical properties of matrices and excitation condition., JOURNAL OF PHYSICAL CHEMISTRY B, vol. 116(32), 9635-9643, 2012具代表性; 任職中研院著作;
2012 Y. Y. Zeng, H.-J. Chen, K. J. Shiau, S.-U. Hung, Y.-S. Wang, C.-C. Wu, Efficient enrichment of phosphopeptides by magnetic TiO2-coated carbon-encapsulated iron nanoparticles , PROTEOMICS, vol. 12 (3), 380-390, 2012任職中研院著作;
2011 O. P. Charkin, N. M. Klimenko, Y.-S. Wang, C.-C. Wang, C.-H. Chen, S.-H. Lin, Theoretical and experimental study of fullerenol molecules and ion C60(OH)24-n(OL)n and C60(OH)24-n(OL)nL+, substituted consecutively by alkali metal atoms L (n = 1 - 24), RUSSIAN JOURNAL OF INORGANIC CHEMISTRY, vol. 56(4), 580-590, 2011任職中研院著作;
2011 C.-H. Hsiao, C.-W. Hong, B.-H. Liu, C. W. Chen, C.-C. Wu, Y.-S. Wang, Comprehensive Molecular Imaging of Photolabile Surface Samples with Synchronized Dual-Polarity Time-of-Flight Mass Spectrometry, RAPID COMMUNICATIONS IN MASS SPECTROMETRY, vol. 25(7), 834-842, 2011具代表性; 任職中研院著作;
2010 Liang, C.-W.; Lee, Y. T.; Chen, C.-H.; Wang, Y.-S., Ionizing nonvolatile samples using laser desorption-proton-transfer reaction with cluster reagent ions, International Journal of Mass Spectrometry, vol. 291 (1-2), 61-66, 2010具代表性; 任職中研院著作;
2010 B.-H. Liu, O. P. Charkin, N. Klemenko, C. W. Chen, Y.-S. Wang*, Initial ionization reaction in matrix-assisted laser/desorption ionization, JOURNAL OF PHYSICAL CHEMISTRY B, vol. 114, 10853-10859, 2010具代表性; 任職中研院著作;
2010 Y.-H. Lai, C.-C. Wang, S.-H. Lin, Y. T. Lee, (Y.-S. Wang*, Solid-Phase Thermodynamic Interpretation of Ion Desorption in Matrix-Assisted Laser Desorption/Ionization, JOURNAL OF PHYSICAL CHEMISTRY B, vol. 114 (43), 13847–13852, 2010具代表性; 任職中研院著作;
2009 Bo-Hong Liu, Yuan Tseh Lee, Yi-Sheng Wang, Incoherent production reactions of positive and negative ions in matrix-assisted laser desorption/ionization, Journal of the American Society for Mass Spectrometry, vol. 20(6), 1078-1086., 2009具代表性; 任職中研院著作;
2009 Chen, H.-K.; Chang, C.-K.; Wu, C.-C.; Huang, M.-C.; Wang, Y.-S., Synchronized dual-polarity electrospray ionization mass spectrometry, JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, vol. 20 (12), 2254-2257., 2009具代表性; 任職中研院著作;
2008 Jessome, L.; Hsu, N.-Y.; Wang, Y.-S.; Chen, C.-H., *, Matrix-assisted laser desorption/ionization mechanism study with dihydroxybenzoic acid isomers as matrices, Rapid Communications in Mass Spectrometry, vol. 22(2), 130-134., 2008任職中研院著作;
2008 Tsai, S.-T.; Chen, C.-H.; Lee, Y. T.; Wang, Y.-S., *, Desorption dynamics of neutral molecules in matrix-assisted laser desorption/ionization., Molecular Physics, vol. 106 (1-2), 239-247, 2008任職中研院著作;
2008 Chang C.-K.;Wu, C.-C.;* Wang, Y.-S.; Chang, H.-C., Selective extraction and enrichment of multiphosphorylated peptides using polyarginine-coated diamond nanoparticles, Analytical Chemistry, vol. 80(10), 3791-3797, 2008任職中研院著作;
2007 S. Sabu, F. C. Yang, Y. S. Wang, W. H. Chen, M. I. Chou, H. C. Chang, C. C. Han, Peptide analysis: Solid phase extraction-elution on diamond combined with atmospheric pressure matrix-assisted laser desorption/ionization-Fourier transform ion cyclotron resonance mass spectrometry, Anal Biochem, vol. 367, 190-200, 2007任職中研院著作;
2007 N. Y. Hsu, W. B. Yang, C. H. Wong, Y. C. Lee, R. T. Lee, Y. S. Wang, C. H. Chen, Matrix-assisted laser desorption/ionization mass spectrometry of polysaccharides with 2',4',6'-trihydroxyacetophenone as matrix, RAPID COMMUNICATIONS IN MASS SPECTROMETRY, vol. 21, 2137-2146, 2007任職中研院著作;
2007 W. C. Chang, L. C. L. Huang, Y.-S. Wang, W.-P. Peng, H.-C. Chang, N. Y. Hsu, W. B. Yang, C. H. Chen, Matrix-assisted laser desorption/ionization (MALDI) mechanism revisited, Analytica Chimica Acta, vol. 582, 1-9, 2007任職中研院著作;
2007 M.-H. Li, S.-T. Tsai, C.-H. Chen, C. W. Chen, Y. T. Lee, Y.-S. Wang, Bipolar Ion Detector Based on Sequential Conversion Reactions, Analytical Chemistry, vol. 79 (4), 1277-1282., 2007具代表性; 任職中研院著作;
2006 S.-T. Tsai, C.-W. Chen, L.-C. Huang, M.-C. Huang, C.-H. Chen, Y.-S. Wang, Simultaneous mass analysis of positive and negative ions using a dual-polarity time-of-flight mass spectrometer, Analytical Chemistry, vol. 78 (22), 7729-7734., 2006具代表性; 任職中研院著作;
2005 O. P. Charkin, N. M. Klimenko, T. P. Nguyen, D. O. Charkin, Y.-S. Wang, S.-C. Wei, H.-C. Chang, S. H. Lin, Theoretical study of fragmentation of heme and its ion with successive removal of carboxymethyl groups, Russian Journal of Inorganic Chemistry, vol. 50 (9), 1398-1407, 2005任職中研院著作;
2004 B. Cage, J. Friedrich, R. B. Little, Y.-S. Wang, M. A. McFarland, C. L. Hendrickson, A. G. Marshall, Wavelength resolved laser-induced fluorescence emission of C6F3H3+ trapped in an ion cyclotron resonance cell, Chemical Physics Letters, vol. 394 (1-3), 188-193, 2004
2004 J. Friedrich, J. M. Fu, C. L. Hendrickson, A. G. Marshall, Y.-S. Wang, Time resolved laser-induced fluorescence of electrosprayed ions confined in a linear quadrupole trap, Review of Scientific Instruments, vol. 75 (11), 4511-4515, 2004