Wang, Yi Sheng 王亦生


[ CV ]




  • Ph.D., Chemistry, National Taiwan University, 2001
  • Postdoctoral Fellow, Institute of Atomic and Molecular Sciences (IAMS), Academia Sinica, 2001-2005
  • Postdoctoral Fellow, National High Magnetic Field Laboratory (NHMFL), Florida State University, U.S.A. 2002-2003
  • Assistant Professor, Genomics Research Center, Academia Sinica, 2005-2011
  • Associate Professor, Genomics Research Center, Academia Sinica, 2011-2019
  • Professor, Genomics Research Center, Academia Sinica, 2019-present
  • Deputy Director, Genomics Research Center, Academia Sinica, 2023-present


  • Outstanding Students Conference Travel Grant, 2000
  • Advanced Abroad Research Fellowship, 2002-2003
  • Outstanding Post-Doctor Researchers Conference Travel Grant, 2002 & 2004


Biophysical Chemistry and Technology Development

Our objective is the advancement of bio-analytical sciences and biophysics in a systematic and complementary manner. Both fundamental biophysical knowledge and technical innovations are essential for resolving critical problems, such as high-throughput diagnoses, accurate quantifications and molecular function examinations. We have studied the physics and chemistries involved in the development of key analytical technologies, and integrated them for express clinical diagnoses. The fundamental study of ionization mechanism of biomolecules is one of our focuses, which enables us to design an effective strategy to improve the detection of carbohydrates. The technologies developed in this laboratory include:

  • Synchronized Dual-Polarity Mass Spectrometry
  • Synchronized Dual-Polarity Imaging Mass Spectrometry
  • Soft Protonation Method for Carbohydrates
  • Ultra-Sensitive Surface Ionization Method for Carbohydrates
  • Structural and Energetic Simulation of Oligosaccharides
  • Nanoparticles for Selective Purification of Phosphoproteins
  Dual-polarity imaging_mass
Dual-polarity imaging mass spectrometer and analytical results of insulin A-chain with matrix on surface. 


基質輔助雷射脫附/游離法 (MALDI) 是蛋白質質譜分析上最重要的離子源,但是其脫附/游離機制至今仍未被完全理解,其中的問題包含了複雜的生物分子解離動力學、基質與生物分子的共晶過程等 等。本實驗室將運用交叉分子束的概念,研究MALDI方法的解離機制,並更進一步研究氣態生物分子的光解離性質,期以此研究突破生物質譜技術的偵測瓶頸。 此外,本實驗室也進行新生物質譜技術研發,未來也將致力於開發質譜儀與其他研究設備的連結介面,期能幫助鑑定疾病並監測治療反應。


  • Yi-Hong Cai, Cheng-Huang Lin, Yi-Sheng Wang, 2023, “Theoretical study of general principle of high-resolution MALDI-linear time-of-flight mass spectrometry in middle to high m/z ranges”, INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 489, 117052, 117052. (SCIE)
  • Lai Yin‐Hung*, Wang Yi‐Sheng*, 2023, “Advances in high‐resolution mass spectrometry techniques for analysis of high mass‐to‐charge ions”, Mass Spectrometry Reviews, 42(6):2426-2445. (SCIE)
  • Chang Ko-Keng, Cai Yi-Hong, Hsiao Chih-Hao, Hsu Cheng-Chih, Wang Yi-Sheng*, 2022, “High-performance miniature linear time-of-flight mass spectrometry as an advantageous tool in a high mass-to-charge range”, The Analyst, 147(18), 4116-4123. (SCIE)
  • C.-H. Hsiao, Y.-H. Lai, S.-Y. Kuo, Y.-H. Cai, C.-H. Lin, Y.-S. Wang*, 2020, “A Dynamic Data Correction Method for Enhancing Resolving Power of Integrated Spectra in Spectroscopic Analysis”, ANALYTICAL CHEMISTRY, 92(19), 12763-12768. (SCIE)
  • Wang Chia-Chen, Wang Yi-Sheng*, 2019, “Effective analysis of degree of polymerization of polysialic acids in mass spectrometry by combining novel sample preparation and dynamic instrument optimization methods”, Carbohydrate Research, 471, 78-84. (SCIE)
  • Ou Yu-Meng, Kuo Shu-Yun, Lee Hsun, Chang Huan-Tsung, Wang Yi-Sheng*, 2018, “An Efficient Sample Preparation Method to Enhance Carbohydrate Ion Signals in Matrix-assisted Laser Desorption/Ionization Mass Spectrometry”, Jove-Journal of Visualized Experiments, 137, e57660. (SCIE)
  • Cai Yi-Hong, Wang Yi-Sheng*, 2018, “Impact of uneven sample morphology on mass resolving power in linear MALDI-TOF mass spectrometry: A comprehensive theoretical investigation”, Journal of Mass Spectrometry, 53(4), 361-368. (SCIE)
  • H. Lee, Y.-H. Lai, Y.-M. Ou, C.-W. Tsao, Y.-J. Jheng, S.-Y. Kuo, H.-T. Chang, Y.-S. Wang*, 2017, “Enhancing carbohydrate ion yield by controlling crystalline structures in matrix-assisted laser desorption/ionization mass spectrometry”, ANALYTICA CHIMICA ACTA, 994, 49-55. (SCIE)
  • C.-C. Wang, Y.-H. Lai, Y.-M. Ou, H.-T. Chang, Y.-S. Wang*, 2016, “Critical Factors Determining Quantification Capability of MALDI-TOF Mass Spectrometry”, PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY A-MATHEMATICAL PHYSICAL AND ENGINEERING SCIENCES, 374(2079), 20150371. (SCIE)
  • Y.-M. Ou, C.-W. Tsao, Y.-H. Lai, H. Lee, H.-T. Chang, Y.-S. Wang*, 2016, “Preparation of homogeneous MALDI samples for quantitative applications”, JOVE-Journal of Visualized experiments, 116, e54409. (SCIE)
  • Urban, P.; Chen, Y.-C.; Wang, Y.-S., 2016, Time-Resolved Mass Spectrometry, 320 pages, London: John Wiley & Sons, Ltd.
  • Chang, K.-K.; Cai, Y.-H.; Chen, C.-H.; Wang, Y.-S.;*, 2016, “Neutralization charge detection method for detecting ions under ambient and liquid phases.”, CHEMICAL COMMUNICATIONS, 52(29), 5187-5189. (SCIE)
  • Y.-H. Lai, Y.-H. Cai, H. Lee, Y.-M. Ou, C.-H. Hsiao, C.-W. Tsao, H.-T. Chang, Y.-S. Wang*, 2016, “Reducing spatial heterogeneity of MALDI samples with Marangoni flows during sample preparation”, JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 27(8), 1314-1321. (SCIE)
  • Cai, Y.-H.; Lai, Y.-H.; Wang, Y.-S.*, 2015, “Coupled space- and velocity-focusing in time-of-flight mass spectrometry – a comprehensive theoretical investigation.”, JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 26(10), 1722-1731. (SCIE)
  • Lai, Y.-H.; Chen, B.-G.; Lee, Y. T.; Wang, Y.-S.;* Lin, S. H.*, 2014, “Contribution of thermal energy to initial ion production of matrix-assisted laser desorption/ionization observed with 2,4,6-trihydroxyacetophenone.”, RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1716-1722. (SCIE)
  • C.-L. Wu, C.-C. Wang, Y.-H. Lai, H. Lee, J.-D. Lin, Y. T. Lee, Y.-S. Wang*, 2013, “Selective enhancement of carbohydrate ion abundances by diamond nanoparticles for mass spectrometric analysis.”, ANALYTICAL CHEMISTRY, 85, 3836-3841. (SCIE)
  • Y.-H. Lai, C.-C. Wang, B.-H. Liu, C. W. Chen, S.-H. Lin, Y. T. Lee, Y.-S. Wang*, 2012, “Analysis of initial reactions of MALDI based on chemical properties of matrices and excitation condition.”, JOURNAL OF PHYSICAL CHEMISTRY B, 116(32), 9635-9643. (SCIE)
  • C.-H. Hsiao, C.-W. Hong, B.-H. Liu, C. W. Chen, C.-C. Wu, Y.-S. Wang, 2011, “Comprehensive Molecular Imaging of Photolabile Surface Samples with Synchronized Dual-Polarity Time-of-Flight Mass Spectrometry”, RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 25(7), 834-842. (SCIE)
  • Y.-H. Lai, C.-C. Wang, S.-H. Lin, Y. T. Lee, Y.-S. Wang*, 2010, “Solid-Phase Thermodynamic Interpretation of Ion Desorption in Matrix-Assisted Laser Desorption/Ionization”, JOURNAL OF PHYSICAL CHEMISTRY B, 114 (43), 13847–13852. (SCIE)
  • B.-H. Liu, O. P. Charkin, N. Klemenko, C. W. Chen, Y.-S. Wang*, 2010, “Initial ionization reaction in matrix-assisted laser/desorption ionization”, JOURNAL OF PHYSICAL CHEMISTRY B, 114, 10853-10859. (SCIE)
  • Liang, C.-W.; Lee, Y. T.; Chen, C.-H.; Wang, Y.-S., 2010, “Ionizing nonvolatile samples using laser desorption-proton-transfer reaction with cluster reagent ions”, International Journal of Mass Spectrometry, 291 (1-2), 61-66. (SCIE)
  • Chen, H.-K.; Chang, C.-K.; Wu, C.-C.; Huang, M.-C.; Wang, Y.-S., 2009, “Synchronized dual-polarity electrospray ionization mass spectrometry”, JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 20 (12), 2254-2257.. (SCIE)
  • Bo-Hong Liu, Yuan Tseh Lee, Yi-Sheng Wang, 2009, “Incoherent production reactions of positive and negative ions in matrix-assisted laser desorption/ionization”, Journal of the American Society for Mass Spectrometry, 20(6), 1078-1086.. (SCIE)
  • M.-H. Li, S.-T. Tsai, C.-H. Chen, C. W. Chen, Y. T. Lee and Y.-S. Wang, 2007, “Bipolar Ion Detector Based on Sequential Conversion Reactions”, Analytical Chemistry, 79 (4), 1277-1282.. (SCIE)
  • S.-T. Tsai, C.-W. Chen, L.-C. Huang, M.-C. Huang, C.-H. Chen and Y.-S. Wang, 2006, “Simultaneous mass analysis of positive and negative ions using a dual-polarity time-of-flight mass spectrometer”, Analytical Chemistry, 78 (22), 7729-7734.. (SCIE)