Chang, Ying Chih 張瑛芝

ChangYC6x7 2研究員





  • B.S., Chemical Engineering, National Taiwan University, Taipei, Taiwan
  • Ph.D., Chemical Engineering, Stanford University, CA, 1998
  • Senior Engineer: Department of Advanced Technology, MMC (Maxtor Corp.), San Jose, CA, USA, 1998
  • Postdoctoral Scientist, Stanford University, CA/ Affymetrix Corp, Santa Clara, CA, USA, 1998-1999
  • Assistant Professor, Department of Chemical Engineering and Materials Science, and Department of Biomedical Engineering, University of California, Irvine, USA, 1999-2003
  • Associate Professor, Genomics Research Center, Academia Sinica, Taipei, Taiwan, 2004-2007
  • Professor, Genomics Research Center, Academia Sinica, Taipei, Taiwan, 2010-present
  • Consulting Professor, Department of Chemical Engineering, Stanford University,  2014-present


Biomimetic Smart Materials and Interfaces

My research focus first aims at the protocol design for the construction of a supramolecular architecture consisting of biomolecules or biomimetic materials.

One of the greatest challenges will be the development of the capability to control the molecular organization from its natural form when interacting with artificial interfaces, thus possibly leading to useful applications that require the incorporation of non-biologically originated materials. The ultimate goal is to establish engineering methodology to fabricate both bio- and non-biological materials with novel functionality inspired by the nature.

For instance, it is known that various biological functions ranging from catalyzing, transporting, to storing energy, etc, arise from the conformational transition of proteins (polypeptides) molecules. Thus, studying the conformational transition of both natural or artificial polypeptide materials might lead to the creation of a novel category of “smart materials” which can be extremely sensitive to the external stimulants from ionic strength, pH, chemicals, lights, heats, and electro-magnetic fields. In our laboratory, we will identify the key factors to establish the protocols, through a series of molecular design, synthesis, functional and structural characterization.

Likewise, various novel supramolecular structures or nanocomposites formed by macromolecules, small chemicals, or nanoparticles, can be readily assembled based on the same design principles.

Rare Cell Isolation, Purification and Maintenance by Smart Surfaces and Microfluidics

We designed and synthesized materials to systematically study the cell-materials interactions which are critical to tissue engineering and fundamental understanding of biological phenomena. By creating new architectures of materials assemblies, we wish to uncover novel properties, which in turn provide unique applications. In the biological applications, we have demonstrated the applicability of these non-biological originated interfaces on controlling cell fate. In particular, we have especially focused on the co-culture systems such as circulating tumor cells in blood, and stem cells in primary cells. These rare cells are important, however, are extremely rare and hard to control. With our available tools, in one case, we are able to demonstrate that the nanostructures of collagen I fibrils are important to control stroma cells, which in turn regulate the self-renewal property of pulmonary stem/ progenitor cells. While there is no stroma cells, the nanostructures of collagen I fibrils become less relevant to the stem cell fate (Huang et al, Biomaterials 2010). In another case, we are able to purify and control fetal liver stem cells in forming suspended spheres, semi-suspended spheres; using the same materials in the presence of lipid bilayers, the maintenance period of stem cells can be doubled. By incorporating the micro-fabricated device, which provide external force fields (mechanically, electrically, and chemically) with both spatial and temporal resolution, we will be able to take one step further in controlling these co-cultured systems in high precision even more efficiently. We will focus on mediating materials-cell interactions to efficiently select, purify, and maintain rare cells such as stem cells in primary culture and circulating tumor cells in blood.

本實驗室主要發展生化奈米材料和其應用。從瞭解生物分子(蛋白、寡核苷酸、寡醣和酯類等)的功能與機制,進而以材料設計的原理產出智慧型,功能性生化有機、無機奈米組織和應用界面。最終目標是開發感測生物有機電子/光學和生物奈米電子機構系統。 近期我們特別致力於發展系列材料以有效應用在純化分離, 及控制血液循環腫瘤細胞及幹細胞的生長與分化。


  • Chen Jia-Yang, Chou Hsu-Huan, Lim Syer Choon, Huang Yen-Jang, Lai Kuan-Chen, Guo Chin-Lin, Tung Chien-Yi, Su Chung-Tsai, Wang Jocelyn, Liu Edward, Han Hsiao-Fen, Yeh Po-Ying, Hu Chun-Mei, Dunn Alexander R., Frank Curtis W., Wu Yi-Chun*, Yang Muh-Hwa*, Chang Ying-Chih*, 2022, “Multiomic characterization and drug testing establish circulating tumor cells as an ex vivo tool for personalized medicine”, iScience, 25(10), 105081. (SCIE)
  • Tsai, Wen-Sy, Tsung-Fu Hung, Jia-Yang Chen, Shu-Huan Huang, and Ying-Chih Chang, 2021, “Early Detection and Dynamic Changes of Circulating Tumor Cells in Transgenic NeuN Transgenic (NTTg) Mice with Spontaneous Breast Tumor Development”, Cancers, 13, 3294. (SCIE)
  • Tzu-Hsien Wu, Cheng-Han Wu, Chun-Jen Huang*, Ying-Chih Chang*, 2021, “Anticlogging Hemofiltration Device for Mass Collection of Circulating Tumor Cells by Ligand-Free Size Selection”, LANGMUIR, 37(11), 3399-3409. (SCIE)
  • Lai Chian-Hui, Tsai Wen-Sy, Yang Muh-Hwa, Chou Teh-Ying, Chang Ying-Chih, 2019, “A two-dimensional immunomagnetic nano-net for the efficient isolation of circulating tumor cells in whole blood”, Nanoscale, 11(44), 21119-21127. (SCIE)
  • Tsai Wen-Sy, Watson Drew, Chang Ying, Hsieh Ben, Shao Hung-Jen, Wu Jen-chia, Lai Jr-Ming, Chang Shih-En, Javey Mana, Mei Rui, Nimgaonkar Ashish, 2019, “Circulating tumor cell count from a blood sample for colorectal cancer (CRC) prevention: A 627-patient prospective study.”, Journal of Clinical Oncology, 37(4_suppl), 485-485. (SCIE)
  • Huang Chun-Jen, Chang Ying-Chih, 2019, “Construction of Cell–Extracellular Matrix Microenvironments by Conjugating ECM Proteins on Supported Lipid Bilayers”, Frontiers in Materials, 6, 39. (SCIE)
  • Tsai Wen-Sy, Nimgaonkar Ashish, Segurado Oscar, Chang Ying, Hsieh Ben, Shao Hung-Jen, Wu Jen-chia, Lai Jr-Ming, Javey Mana, Watson Drew, Mei Rui, 2019, “Prospective clinical study of circulating tumor cells for colorectal cancer screening.”, Journal of Clinical Oncology, 36(4_suppl), 556-556. (SCIE)
  • Yu Chia-Cheng, Chen Yi-Wen, Yeh Po-Ying, Hsiao Yu-Sheng, Lin Wei-Ting, Kuo Chiung-Wen, Chueh Di-Yen, You Yun-Wen, Shyue Jing-Jong, Chang Ying-Chih, Chen Peilin, 2019, “Random and aligned electrospun PLGA nanofibers embedded in microfluidic chips for cancer cell isolation and integration with air foam technology for cell release”, Journal of Nanobiotechnology, 17, 31. (SCIE)
  • Tsai Yu-Lin, Yeh Po-Ying, Huang Chun-Jen, Guo Chin-Lin, Chang Ying-Chih, 2019, “Scalable Multilayer Cell Collector to Capture Circulating Tumor Cells with an Unlimited Volume Capacity”, ACS Biomaterials Science & Engineering, 5(6), 2725-2731. (SCIE)
  • Li CF, Chen JY, Ho YH, Hsu WH, Wu LC, Lan HY, Hsu DS, Tai SK, Chang YC, Yang MH, 2019, “Snail-induced claudin-11 prompts collective migration for tumour progression.”, Nature Cell Biology, 21(2), 251-262. (SCIE)
  • Yang Chih-Tsung, Wu Jen-Chia, Chang Ying-Chih, 2018, “Facile Synthesis of High Molecular Weight Polypeptides via Surface-Initiated Vapor Deposition Polymerization”, Polymer Preprints, Version 1.
  • Pang SEE-TONG, Chang Ying-Hsu, Lin Po-Hung, Chang Ying, Watson Drew, Segurado Oscar, Lu Si-Hong, Wu Jen-chia, Lai Jr-Ming, Shao Hung-Jen, Chang Shih-En, Hsieh Ben, Javey Mana, Mei Rui, 2018, “Prospective clinical study of a prostate cancer (PCa) rule-out blood test for PSA gray zone patients using a sensitive circulating tumor cell assay.”, Journal of Clinical Oncology, 36(6_suppl), 143-143. (SCIE)
  • Po-Ying Yeh, Yih-Ruey Chen, Chien-Fang Wang, and Ying-Chih Chang*, 2017, “Promoting Multivalent Antibody−Antigen Interactions by Tethering Antibody Molecules on a PEGylated Dendrimer-Supported Lipid Bilayer”, BIOMACROMOLECULES, 19(2), 426-437. (SCIE)
  • Chian-Hui Lai, Syer Choon Lim, Liang-Chun Wu, Chien-Fang Wang, Wen-Sy Tsai, Han-Chung Wu and Ying-Chih Chang*, 2017, “Site-specific antibody modification and immobilization on a microfluidic chip to promote the capture of circulating tumor cells and microemboli”, CHEMICAL COMMUNICATIONS, 53(29), 4152-4155. (SCIE)
  • Ming-Chu Chang, Ying-Chih Chang, Yung-Ming Jeng, Ching-Yao Yang, Yu-Wen Tien, Shih-Hung Yang, Eva Y.H.P. Lee, Wen-Hwa Lee, Yu-Ting Chang, 2016, “Circulating tumor cluster is a prognostic marker in patients with pancreatic ductal adenocarcinoma”, PANCREATOLOGY, 16(4), S53-S53. (SCIE)
  • Yu Wen Tien, Hsun-Chuan Kuo, Be-Ing Ho, Ming-Chu Chang, Yu-Ting Chang, Mei-Fang Cheng, Huai-Lu Chen, Ting-Yung Liang, Chien-Fang Wang, Chia-Yi Huang, Jin-Yuh Shew, Ying Chih Chang, Eva YHP Lee and Wen-Hwa Lee, 2016, “A High Circulating Tumor Cell Count in Portal Vein Predicts Liver Metastasis From Periampullary or Pancreatic Cancer A High Portal Venous CTC Count Predicts Liver Metastases”, MEDICINE, 95(16), e3407. (SCIE)
  • Wen-Sy Tsai, Jinn-Shiun Chen, Hung-Jen Shao, Jen-Chia Wu, Jr-Ming Lai, Si-Hong Lu, Tsung-Fu Hung, Yen-Chi Chiu, Jeng-Fu You, Pao-Shiu Hsieh, Chien-Yuh Yeh, Hsin-Yuan Hung, Sum-Fu Chiang, Geng-Ping Lin, Reiping Tang and Ying-Chih Chang* , 2016, “Circulating Tumor Cell Count Correlates with Colorectal Neoplasm Progression and Is a Prognostic Marker for Distant Metastasis in Non-Metastatic Patients”, Scientific Reports, 6, 24517. (SCIE)
  • Ming-Chu Chang, Yu-Ting Chang*, Jia-Yang Chen, Yung-Ming Jeng, Ching-Yao Yang, Yu-Wen Tien, Shih-Hung Yang, Huai-Lu Chen, Ting-Yuan Liang, Chien-Fang Wang, Eva Y.H.P. Lee, Ying-Chih Chang, and Wen-Hwa Lee*, 2016, “Clinical Significances of Circulating Tumor Microemboli as a Prognostic Marker in Patients with Pancreatic Ductal Adenocarcinoma”, CLINICAL CHEMISTRY, 62(3), 505-513. (SCIE)
  • Jia-Yang Chen, Wen-Sy Tsai, Hung-Jen Shao, Jen-Chia Wu, Jr-Ming Lai, Si-Hong Lu, Tsung-Fu Hung, Chih-Tsung Yang, Liang-Chun Wu, Jinn-Shiun Chen, Wen-Hwa Lee, Ying-Chih Chang, 2016, “Sensitive and Specific Biomimetic Lipid Coated Microfluidics to Isolate Viable Circulating Tumor Cells and Microemboli for Cancer Detection”, PLoS One, 11(3), e0149633. (SCIE)
  • Si-Hong Lu, Wen-Sy Tsai, Ying-Hsu Chang, Teh-Ying Chou, See-Tong Pang, Po-Hung Lin, Chun-Ming Tsai and Ying-Chih Chang* , 2016, “Identifying Cancer Origin Using Circulating Tumor Cells”, Cancer Biology & Therapy, 17(4), 430-438. (SCIE)
  • See-Tong Pang, Ying-Hsu Chang, Po-Hung Lin, Ying-Chih Chang,Yow-sien Lin, Ben Hsieh, Chung-Yi Liu, Cheng-Keng Chuang, Ritu Kamal, Rui Mei*, 2016, “A Novel biomarker for prostate cancer detection in patient with gray zone PSA level”, Urological Science, 27(2), S1-S2.
  • Chih-Tsung Yang, Yuli Wang, Curtis W. Frank* and Ying-Chih Chang*, 2015, “Chemoresponsive surface-tethered polypeptide brushes based on switchable secondary conformations”, RSC Advances, 5(105), 86113-86119. (SCIE)
  • Lee, I-Chi; Liu, Yung-Chiang; Tsai, Hsuan–Ang; Shen, Chia-Ning; Chang, Ying-Chih*, 2014, “Promoting the selection and maintenance of fetal liver stem/ progenitor cell colonies by layer-by-layer polypeptide tethered supported lipid bilayer”, ACS Applied Materials & Interfaces, 6, 20654-20663. (SCIE)
  • Chun-Jen Huang*, Lin-Chuan Wang, Jing-Jong Shyue, and Ying-Chih Chang, 2014, “Developing Antifouling Biointerfaces Based on Bioinspired Zwitterionic Dopamine through pH-Modulated Assembly”, LANGMUIR, 30(42), 12638-12646. (SCIE)
  • Jr-Ming Lai, Hung-Jen Shao, Jen-Chia Wu, Si-Hong Lu, Ying-Chih Chang*, 2014, “Efficient elusion of viable adhesive cells from a microfluidic system by air foam”, Biomicrofluidics, 8, 052001. (SCIE)
  • Chun-Jen Huang*, Jhong-Li, Ying-Chih Chang*, 2014, “Natural zwitterionic organosulfurs as surface ligands for antifouling and responsive properties”, Biointerphases, 9(2), 029010-6. (SCIE)
  • Chun-Jen Huang,* Ying-Chih Chang*, 2014, “In Situ Surface Tailoring with Zwitterionic Carboxybetaine Moieties on Self-Assembled Thin Film for Antifouling Biointerfaces”, Materials, 7(1), 130-142. (SCIE)
  • I-Chi Lee*, Tzu-Lin Lo, Tai-Horng Young, Yi-Chen Li, Nelson G. Chen, Chung-Hsuan Chen, Ying-Chih Chang*, 2014, “Differentiation of Neural Stem/Progenitor Cells Using Low Intensity Ultrasound”, ULTRASOUND IN MEDICINE AND BIOLOGY, 40(9), 2195-2206. (SCIE)
  • Jen-Chia Wu, Po-Yuan Tseng, Wen-Sy Tsai, Mei-Ying Liao, Si-Hong Lu, Curtis W. Frank, Jinn-Shiun Chen, Han-Chung Wu*, Ying-Chih Chang*, 2013, “Antibody conjugated supported lipid bilayer for capturing and purification of viable tumor cells in blood for subsequent cell culture”, BIOMATERIALS, 34, 5191-5199. (SCIE)
  • Hsuan-Ang Tsai , Chia-Ning Shen , and Ying-Chih Chang*, 2012, “The Use of Surface Properties to Control the Growth and Differentiation of Mouse Fetal Liver Stem/Progenitor Cell Colonies”, BIOMACROMOLECULES, 13 (11), 3483-3493. (SCIE)
  • Po-Yuan Tseng and Ying-Chih Chang*, 2012, “Tethered Fibronectin Liposomes on Supported Lipid Bilayers as a Pre-Packaged Controlled-Release Platform for Cell-Based Assays”, BIOMACROMOLECULES, 13(8), 2254-2262. (SCIE)
  • Jen-Chia Wu, Chia-Chun Chen, Kuei-Hsien Chen, and Ying-Chih Chang*, 2011, “Controlled Growth of Aligned Alpha-Helical Polypeptide Brushes for Tunable Electrical Conductivity”, Applied Physics Letters, 98, 133304. (SCIE)
  • Chun-Jen Huang, Yi-Lun Chien, Tai-Yen Ling, Huan-Chien Cho, John Yu*, and Ying-Chih Chang*, 2010, “The Influence of Collagen Film Nanostructure on Pulmonary Stem Cells and Collagen-Stromal Cell Interactions”, BIOMATERIALS, 31(32), 8271-8280. (SCIE)
  • Chih-Tsung Yang, Yuli Wang, Ying-Chih Chang*, 2010, “Effect of Solvents and Temperature on the COnformation of Poly(beta-benzyl-L-aspartate) Brushes”, BIOMACROMOLECULES, 11(5), 1308-1313. (SCIE)
  • Chun-Jen Huang, Po-Yuan Tseng, and Ying-Chih Chang*, 2010, “Effects of Extracellular Matrix Protein Functionalized Fluid Membrane on Cell Adhesion and Matrix Remodeling”, BIOMATERIALS, 31, 7183-7195. (SCIE)
  • Hsuan-Ang Tsai, I-Chi Lee, Ruei-Ren Wu, Hsiao-Yuan Chang, Chia-Ning Shen*, Ying-Chih Chang*, 2010, “Selection, Enrichment, and Maintenance of Self-Renewal Liver Stem/Progenitor Cells Utilizing Polypeptide Polyelectrolyte Multilayer Films”, BIOMACROMOLECULES, 11(4), 994-1001. (SCIE)
  • Chun-Jen Huang, Nam-Joon Cho, Chih-Jung Hsu, Curtis W. Frank*, Ying-Chih Chang*, 2010, “Type I Collagen-Functionalized Supported Lipid Bilayer as a Cell Culture Platform”, BIOMACROMOLECULES, 11(5), 1231-1240. (SCIE)
  • Di-Yan Wang, Tzu-Shen Teng, Yi-Chou Wu, Yi-Cheng Lee, Kuei-Hsien Chen, Chung-Hsuan Chen, Ying-Chih Chang*, Chia-Chun Chen*, 2009, “Silver-Nanoparticle-Conjugated Polypeptide Brushes for Surface-Enhanced Raman Scattering”, JOURNAL OF PHYSICAL CHEMISTRY C, 113(31), 13498-13504. (SCIE)
  • Chen CP, Ganguly A, Wang CH, Hsu CW, Chattopadhyay S, Hsu YK, Chang YC, Chen KH, Chen LC, 2009, “Label-free dual sensing of DNA molecules using GaN nanowires.”, ANALYTICAL CHEMISTRY, 81(1), 36-42. (SCIE)
  • Jen-Chia Wu, Yuli Wang, Chia-Chun Chen*, and Ying-Chih Chang*, 2008, “Biomimetic Synthesis of Silica Films Directed by Polypeptide Brushes”, CHEMISTRY OF MATERIALS, 20, 6148-6156. (SCIE)
  • Chih-Tsung Yang, Yuli Wang, and Ying-Chih Chang*, 2008, “Controlled molecular organization of surface macromolecular assemblies based on stimuli-responsive polypeptide brushes.”, BIOMACROMOLECULES, 10(1), 58-65. (SCIE)
  • Jun Li, and Ying-Chih Chang*, 2005, “Preparation of End-Grafted Polymer Brushes by Nitroxide-Mediated Free Radical Polymerization of Vaporized Vinyl Monomers”, LANGMUIR, 21, 9562-9567. (SCIE)
  • Y.L. Wang and Y. C. Chang*, 2003, “Patterning of Polypeptide Thin Films by the Combination of Surface-Initiated Vapor Deposition Polymerization and Photolithography”, ADVANCED MATERIALS, 15(4), 290-293. (SCIE)
  • Y. L. Wang and Y. C. Chang*, 2003, “Preparation of Unidirectional End-Grafted alpha-Helical Polypeptides by Solvent Quenching”, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 125, 6376-6377. (SCIE)
  • Y.L. Wang and Y.C. Chang*, 2003, “Synthesis and Conformational Transition of Surface Tethered Polypeptide: Poly (L-glutamic acid)”, MACROMOLECULES, 36, 6503-6510. (SCIE)
  • Y. L. Wang and Y.C. Chang*, 2003, “Synthesis and Conformational Transition of Surface Tethered Polypeptide: Poly L-lysine”, MACROMOLECULES, 36, 6511-6518. (SCIE)
  • Y. Wang and Y. -C. Chang*, 2002, “Grafting of Homo- Block Co-polypeptides on Solid Substrates by an Improved Surface-Initiated Vapor Deposition Polymerization”, LANGMUIR, 18, 9859-9866. (SCIE)