Wang, Yi Sheng

Researcher / Professor





  • Ph.D., Chemistry, National Taiwan University, 2001
  • Postdoctoral Fellow, Institute of Atomic and Molecular Sciences (IAMS), Academia Sinica, 2001-2005
  • Postdoctoral Fellow, National High Magnetic Field Laboratory (NHMFL), Florida State University, U.S.A. 2002-2003
  • Assistant Professor, Genomics Research Center, Academia Sinica, 2005-2011
  • Associate Professor, Genomics Research Center, Academia Sinica, 2011-2019
  • Research Fellow, Genomics Research Center, Academia Sinica, 2019-present


  • Outstanding Students Conference Travel Grant, 2000
  • Advanced Abroad Research Fellowship, 2002-2003
  • Outstanding Post-Doctor Researchers Conference Travel Grant, 2002 & 2004


Biophysical Chemistry and Technology Development

Our objective is the advancement of bio-analytical sciences and biophysics in a systematic and complementary manner. Both fundamental biophysical knowledge and technical innovations are essential for resolving critical problems, such as high-throughput diagnoses, accurate quantifications and molecular function examinations. We have studied the physics and chemistries involved in the development of key analytical technologies, and integrated them for express clinical diagnoses. The fundamental study of ionization mechanism of biomolecules is one of our focuses, which enables us to design an effective strategy to improve the detection of carbohydrates. The technologies developed in this laboratory include:

  • Synchronized Dual-Polarity Mass Spectrometry
  • Synchronized Dual-Polarity Imaging Mass Spectrometry
  • Soft Protonation Method for Carbohydrates
  • Ultra-Sensitive Surface Ionization Method for Carbohydrates
  • Structural and Energetic Simulation of Oligosaccharides
  • Nanoparticles for Selective Purification of Phosphoproteins
  Dual-polarity imaging_mass
Dual-polarity imaging mass spectrometer and analytical results of insulin A-chain with matrix on surface. 



  • Wang Chia-Chen, Wang Yi-Sheng*, accepted, “Effective analysis of degree of polymerization of polysialic acids in mass spectrometry by combining novel sample preparation and dynamic instrument optimization methods”, Carbohydrate Research, 471, 78-84. (SCIE) (IF: 1.841; SCIE ranking: 82.5%,61.4%,46.5%)
  • C.-H. Hsiao, Y.-H. Lai, S.-Y. Kuo, Y.-H. Cai, C.-H. Lin, Y.-S. Wang*, 2020, “A Dynamic Data Correction Method for Enhancing Resolving Power of Integrated Spectra in Spectroscopic Analysis”, ANALYTICAL CHEMISTRY, 92 (19), 12763-12768. (SCIE, SSCI) (IF: 6.785; SCIE ranking: 8.1%)
  • Ou Yu-Meng, Kuo Shu-Yun, Lee Hsun, Chang Huan-Tsung, Wang Yi-Sheng*, 2018, “An Efficient Sample Preparation Method to Enhance Carbohydrate Ion Signals in Matrix-assisted Laser Desorption/Ionization Mass Spectrometry”, Jove-Journal of Visualized Experiments, 137, e57660. (SCIE) (IF: 1.163; SCIE ranking: 63.4%)
  • Cai Yi-Hong, Wang Yi-Sheng*, 2018, “Impact of uneven sample morphology on mass resolving power in linear MALDI-TOF mass spectrometry: A comprehensive theoretical investigation”, Journal of Mass Spectrometry, 53(4), 361-368. (SCIE) (IF: 1.671; SCIE ranking: 79.2%,69.8%,57.1%)
  • H. Lee, Y.-H. Lai, Y.-M. Ou, C.-W. Tsao, Y.-J. Jheng, S.-Y. Kuo, H.-T. Chang, Y.-S. Wang*, 2017, “Enhancing carbohydrate ion yield by controlling crystalline structures in matrix-assisted laser desorption/ionization mass spectrometry”, ANALYTICA CHIMICA ACTA, 994, 49-55. (SCIE) (IF: 5.977; SCIE ranking: 11.6%)
  • C.-C. Wang, Y.-H. Lai, Y.-M. Ou, H.-T. Chang, Y.-S. Wang*, 2016, “Critical Factors Determining Quantification Capability of MALDI-TOF Mass Spectrometry”, PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY A-MATHEMATICAL PHYSICAL AND ENGINEERING SCIENCES, 374(2079), 20150371. (SCIE) (IF: 3.275; SCIE ranking: 31%)
  • Y.-M. Ou, C.-W. Tsao, Y.-H. Lai, H. Lee, H.-T. Chang, Y.-S. Wang*, 2016, “Preparation of homogeneous MALDI samples for quantitative applications”, JOVE-Journal of Visualized experiments, 116, e54409. (SCIE) (IF: 1.163; SCIE ranking: 63.4%)
  • Urban, P.; Chen, Y.-C.; Wang, Y.-S., 2016, Time-Resolved Mass Spectrometry, 320 pages, London: John Wiley & Sons, Ltd.
  • Chang, K.-K.; Cai, Y.-H.; Chen, C.-H.; Wang, Y.-S.;*, 2016, “Neutralization charge detection method for detecting ions under ambient and liquid phases.”, CHEMICAL COMMUNICATIONS, 52(29), 5187-5189. (SCIE) (IF: 5.996; SCIE ranking: 19.2%)
  • Y.-H. Lai, Y.-H. Cai, H. Lee, Y.-M. Ou, C.-H. Hsiao, C.-W. Tsao, H.-T. Chang, Y.-S. Wang*, 2016, “Reducing spatial heterogeneity of MALDI samples with Marangoni flows during sample preparation”, JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 27(8), 1314-1321. (SCIE) (IF: 3.255; SCIE ranking: 44%,48.1%,14.3%,26.7%)
  • Cai, Y.-H.; Lai, Y.-H.; Wang, Y.-S.*, 2015, “Coupled space- and velocity-focusing in time-of-flight mass spectrometry – a comprehensive theoretical investigation.”, JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 26(10), 1722-1731. (SCIE) (IF: 3.255; SCIE ranking: 44%,48.1%,14.3%,26.7%)
  • Lai, Y.-H.; Chen, B.-G.; Lee, Y. T.; Wang, Y.-S.;* Lin, S. H.*, 2014, “Contribution of thermal energy to initial ion production of matrix-assisted laser desorption/ionization observed with 2,4,6-trihydroxyacetophenone.”, RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1716-1722. (SCIE) (IF: 2.2; SCIE ranking: 50%,59.7%,35.7%)
  • C.-L. Wu, C.-C. Wang, Y.-H. Lai, H. Lee, J.-D. Lin, Y. T. Lee, Y.-S. Wang*, 2013, “Selective enhancement of carbohydrate ion abundances by diamond nanoparticles for mass spectrometric analysis.”, ANALYTICAL CHEMISTRY, 85, 3836-3841. (SCIE) (IF: 6.785; SCIE ranking: 8.1%)
  • Y.-H. Lai, C.-C. Wang, B.-H. Liu, C. W. Chen, S.-H. Lin, Y. T. Lee, Y.-S. Wang*, 2012, “Analysis of initial reactions of MALDI based on chemical properties of matrices and excitation condition.”, JOURNAL OF PHYSICAL CHEMISTRY B, 116(32), 9635-9643. (SCIE) (IF: 2.857; SCIE ranking: 49.7%)
  • C.-H. Hsiao, C.-W. Hong, B.-H. Liu, C. W. Chen, C.-C. Wu, Y.-S. Wang, 2011, “Comprehensive Molecular Imaging of Photolabile Surface Samples with Synchronized Dual-Polarity Time-of-Flight Mass Spectrometry”, RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 25(7), 834-842. (SCIE) (IF: 2.2; SCIE ranking: 50%,59.7%,35.7%)
  • Y.-H. Lai, C.-C. Wang, S.-H. Lin, Y. T. Lee, Y.-S. Wang*, 2010, “Solid-Phase Thermodynamic Interpretation of Ion Desorption in Matrix-Assisted Laser Desorption/Ionization”, JOURNAL OF PHYSICAL CHEMISTRY B, 114 (43), 13847–13852. (SCIE) (IF: 2.857; SCIE ranking: 49.7%)
  • B.-H. Liu, O. P. Charkin, N. Klemenko, C. W. Chen, Y.-S. Wang*, 2010, “Initial ionization reaction in matrix-assisted laser/desorption ionization”, JOURNAL OF PHYSICAL CHEMISTRY B, 114, 10853-10859. (SCIE) (IF: 2.857; SCIE ranking: 49.7%)
  • Liang, C.-W.; Lee, Y. T.; Chen, C.-H.; Wang, Y.-S., 2010, “Ionizing nonvolatile samples using laser desorption-proton-transfer reaction with cluster reagent ions”, International Journal of Mass Spectrometry, 291 (1-2), 61-66. (SCIE) (IF: 2.09; SCIE ranking: 51.4%,38.1%)
  • Chen, H.-K.; Chang, C.-K.; Wu, C.-C.; Huang, M.-C.; Wang, Y.-S., 2009, “Synchronized dual-polarity electrospray ionization mass spectrometry”, JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 20 (12), 2254-2257.. (SCIE) (IF: 3.255; SCIE ranking: 44%,48.1%,14.3%,26.7%)
  • Bo-Hong Liu, Yuan Tseh Lee, Yi-Sheng Wang, 2009, “Incoherent production reactions of positive and negative ions in matrix-assisted laser desorption/ionization”, Journal of the American Society for Mass Spectrometry, 20(6), 1078-1086.. (SCIE) (IF: 3.255; SCIE ranking: 44%,48.1%,14.3%,26.7%)
  • M.-H. Li, S.-T. Tsai, C.-H. Chen, C. W. Chen, Y. T. Lee and Y.-S. Wang, 2007, “Bipolar Ion Detector Based on Sequential Conversion Reactions”, Analytical Chemistry, 79 (4), 1277-1282.. (SCIE) (IF: 6.785; SCIE ranking: 8.1%)
  • S.-T. Tsai, C.-W. Chen, L.-C. Huang, M.-C. Huang, C.-H. Chen and Y.-S. Wang, 2006, “Simultaneous mass analysis of positive and negative ions using a dual-polarity time-of-flight mass spectrometer”, Analytical Chemistry, 78 (22), 7729-7734.. (SCIE) (IF: 6.785; SCIE ranking: 8.1%)