Yang, Wen-Bin 楊文彬

YangWB_6x7研究技師


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02-27871264
[ CV ]
 

 

 

EDUCATION AND POSITIONS HELD:

  • B. S., in Chemistry, ChungYuanChristianUniversity, Taiwan R.O.C., 1989–1992
  • M. S., Department of Chemistry, National Chung Hsing University, 1992–1994
  • Ph.D., Department of Chemistry, National Taiwan University, 1994–1998
  • Postdoctoral Research Fellow, Institute of Biological Chemistry, Academia Sinica, 1998–2002
  • Assistant Professor, Graduate Institute of Pharmacognosy Science, Taipei Medical University, 2002–2003
  • Assistant Research Fellow, GenomicsResearchCenter, Academia Sinica, 2003–2013
  • Associate Research Specialist, Genomics Research Center, Academia Sinica, Taiwan, 2013-2018
  • Senior Research Specialist, Genomics Research Center, Academia Sinica, 2018-present

HONORS:

  • Fellowship of Ministry of Education and NSC, 1992–1994
  • Graduate Fellowship of National Science Council (NSC), 1994–1998
  • The best research paper in Journal of the Chinese Chemical Society, 2000
  • Postdoctoral fellowship in Academia Sinica, 1998–2002

RESEARCH INTERESTS:

The main interest of our group is the structural determination of polysaccharides in medicinal herbs. Polysaccharides such as Ganoderma lucidum (靈芝), Dendrobium houshanens (霍山石斛) and Cordyceps sinensis (冬蟲夏草) were isolated to study their biological activities and structural SAR. For characterization of polysaccharides we used HPAEC, GC-MS, CE, NMR, glycanase and MALDI-MS spectrum to investigate structures of oligosaccharides and polysaccharides. We also developed some convenient and efficient methods to label poly-, oligosaccharides which can be useful on purification and detection. A series of aldose-imidazole and aldose-bis-indole derivatives with fluorescence active was synthesized for composition analysis and chiral resoluation of monosaccharides from medicinal herbs. Research work in Yang laboratory covers several areas:

  • Organic chemistry (有機化學)
  • Carbohydrates chemistry (醣化學)
  • Natural product chemistry (天然物化學)
  • Saccharides analytic chemistry (醣分析化學)
  • Asymmetric catalytic reaction (不對稱催化反應)

研究靈芝多醣調節免疫系統及抗癌活性的生化功能及多醣體之化學組成,利用化學及酵素方法降解高分子量的靈芝多醣,純化並鑑定出較小分子量的靈芝多醣。設計和合成與人類疾病有關的病毒或細菌之蛋白分解酵素抑制劑,以有機磷衍生的縮氨酸(peptides)類似物來仿傚酵素活性中心的過渡狀態,達到抑制蛋白分解酵素的目的。

SELECTED PUBLICATIONS:

  • S.-M. Liang, Y.-P. Chen, W.-B. Yang, C.-H. Wong, accepted, “Reishi F3 subfraction polysaccharides and methods of using same”, Patent Application, pp. US 2009/0263897 A1.
  • Jan JT, Cheng TR, Juang YP, Ma HH, Wu YT, Yang WB, Cheng CW, Chen X, Chou TH, Shie JJ, Cheng WC, Chein RJ, Mao SS, Liang PH, Ma C, Hung SC, Wong CH, 2021, “Identification of existing pharmaceuticals and herbal medicines as inhibitors of SARS-CoV-2 infection.”, Proceedings of the National Academy of Sciences of the United States of America, 118 (5), e2021579118. (SCIE)
  • Yang WB*; Wang SH; Chen YT, 2020, Applications of NMR Spectroscopy, 1-24 pages, Sharjah, United Arab Emirates: Bentham Science Publishers.
  • Hung WT, Chen YT, Chen CH, Lee YC, Fang JM, Yang WB*, 2020, “Flow Chemistry System for Carbohydrate Analysis by Rapid Labeling of Saccharides after Glycan Hydrolysis.”, SLAS technology, 25(4), 356-366. (SCIE)
  • M. H. Wu, Y. C. Lee, W. J. Tsai, W. B. Yang, Y. C. Chen, K. A. Chuang, J. F. Liao, C. C. Wang, Y. C. Kuo, 2010, “Characterized polysaccharides from black soybean induce granulocyte colony-stimulated factor gene expression in a phosphoinositide 3-kinase-dependent manner”, IMMUNOLOGICAL INVESTIGATIONS, 39, 1-23. (SCIE)
  • C. Y. Lai, J. T. Hung, H. H. Lin, A. L. Yu, S. H. Chen, Y. C. Tsai, L. E. Shao, W. B. Yang, J. Yu, 2010, “Immunomodulatory and adjuvant activities of a polysaccharide extract of Ganoderma lucidum in vivo and in vitro”, VACCINE, 28(31),4945-4954. (SCIE)
  • C. Lin, W. T. Hung, C. Y. Kuo, K. S. Liao, Y. C. Liu, W. B. Yang, 2010, “Iodine-catalyzed oxidative condensation of aldoses with diamine on aldo-naphthimidazoles synthesis for carbohydrate analysis”, MOLECULES, 15(3), 1340-1353. (SCIE)
  • C. C. Chang, A. F. Ku, Y. Y. Tseng, W. B. Yang, J. M. Fang, C. H. Wong, 2010, “6,8-Di-C-glycosyl flavonoids from Dendrobium huoshanense”, JOURNAL OF NATURAL PRODUCTS, 73(2), 229-232. (SCIE)
  • T. L. Hsu, S. C. Cheng, W. B. Yang, S. W. Chin, B. H. Chen, M. T. Huang, S. L. Hsieh, C. H. Wong, 2009, “Profiling carbohydrate-receptor interaction with recombinant innate immunity receptor-Fc fusion proteins”, JOURNAL OF BIOLOGICAL CHEMISTRY, 284(50), 34479-34489. (SCIE)
  • Yves. S. Y. Hsieh, S. F. Liao, W. B. Yang, 2009, “Biologically active polysaccharides in medicinal plants”, NEW ZEALAND JOURNAL OF FORESTRY SCIENCE, 39, 217-223. (SCIE)
  • M.H. Chuang , S.H. Chiou, C.H. Huang, W.B. Yang, C.H. Wong, 2009, “The lifespan-promoting effect of acetic acid and Reishi polysaccharide”, BIOORGANIC & MEDICINAL CHEMISTRY, 17(22), 7831-7841. (SCIE)
  • Y. T. Huang, S. Y. Lyu, P. H. Chuang, N. S. Hsu, Y. S. Li, H. C. Chan, Y. C. Liu, C. J. Wu, W. B. Yang, T. L. Li, 2009, “In vitro characterization of enzymes involved in the synthesis of nonproteinogenic residue (2S,3S)-beta-methylphenylalanine in glycopeptide antibiotic mannopeptimycin”, CHEMBIOCHEM, 10(15), 2480-2487. (SCIE)
  • C.-H. Wong, M.-H. Chuang, W.-B. Yang, S.-H. Chiou, 2009, “Longevity-Promoting Effects of Acetic Acid and Reishi Polysaccharide”, PCT Patent Application, pp. No. PCT/US09/40879.
  • C.-H. Wong, M.-H. Chuang, W.-B. Yang, S.-H. Chiou, 2009, “Longevity-Promoting Effects of Acetic Acid and Reishi Polysaccharide”, US Patent Application, pp. No. 12/425,319.
  •  C.-H. Wong, J.-M. Fang, W.-B. Yang, T.-J. R. Cheng, Y. S.-Y. Hsieh, C. Chien, C.-C. Lin, H.-Y. Wen, 2009, “Structure and Bioactivity of the Polysaccharides in Medicinal Plant Dendrobium huoshanense”, PCT Patent Application, pp. No. PCT/US2009/033940.
  •  C.-H. Wong, J.-M. Fang, W.-B. Yang, T.-J. R. Cheng, Y. S.-Y. Hsieh, C. Chien, C.-C. Lin, H.-Y. Wen, 2009, “Structure and Bioactivity of the Polysaccharides in Medicinal Plant Dendrobium huoshanense”, Taiwan Patent Application, pp. No. 098104560.
  •  C.-H. Wong, J.-M. Fang, W.-B. Yang, T.-J. R. Cheng, Y. S.-Y. Hsieh, C. Chien, C.-C. Lin, H.-Y. Wen, 2009, “Structure and Bioactivity of the Polysaccharides in Medicinal Plant Dendrobium huoshanense”, US Patent Application, pp. No. 12/378,263.
  • Shao, Y. M.; Yang, W. B.; Kuo, T. H.; Tsai, K. C.; Lin, C. H.; Yang, A. S.; Liang, P. H.; Wong, C. H., 2008, “Design, Synthesis, and Evaluation of Trifluoromethyl Ketones as Inhibitors of SARS-CoV 3CL Protease”, Bioorganic & Medicinal Chemistry, 16, 4652-4660. (SCIE)
  • M. L. Chen, A. K. Adak, N. C. Yeh, W. B. Yang, Y. J. Chuang, C. H. Wong, K. C. Hwang, J. R. Hwu, S. L. Hsieh, C. C. Lin, 2008, “Fabrication of an oriented Fc-fused lectin microarray through boronate formation”, Angewandte Chemie-International Edition, 47, 8627-8630. (SCIE)
  • Y. S. Hsieh, C. Chien, S. K. Liao, S. F. Liao, W. T. Hung, W. B. Yang, C. C. Lin, T. J. Cheng, C. C. Chang, J. M. Fang and C. H. Wong, 2008, “Structure and bioactivity of the polysaccharides in medicinal plant Dendrobium huoshanense”, Bioorganic & Medicinal Chemistry, 16, 6054-6068. (SCIE)
  • W. T. Hung.; S. H. Wang.; C. H. Chen.; W. B. Yang, 2008, “Structure determination of beta-glucans from Ganoderma lucidum with matrix-assisted laser desorption/ionization (MALDI) mass spectrometry”, Molecules, 13, 1538-1550. (SCIE)
  • C. Lin, P. T. Lai, K. S. Liao, W. T. Hung, W. B. Yang and J. M. Fang, 2008, “Using molecular iodine in direct oxidative condensation of aldoses with diamines: An improved synthesis of aldo-benzimidazoles and aldo-naphthimidazoles for carbohydrate analysis”, J. Org. Chem, 73, 3848-3853. (SCIE)
  • K. F. Hua, H. Y. Hsu, L. K. Chao, S. T. Chen, W. B. Yang, J. Hsu and C. H. Wong, 2007, “Ganoderma lucidum Polysaccharides Enhance CD14 Endocytosis of LPS and Promote TLR4 Signal Transduction of Cytokine Expression”, Journal of Cellular Physiology, 212, 537-550. (SCIE)
  • K. C. Cheng, H. C. Huang, J. H. Chen, J. W. Hsu, H. C. Cheng, C. H. Ou1, W. B. Yang, S. T. Chen, C. H. Wong and H. F. Juan, 2007, “Ganoderma lucidum polysaccharides in human monocytic leukemia cells: from gene expression to network construction”, BMC Genomics, 8, 411. (SCIE)
  • W. C. Chang, L. C. L. Huang, Y. S. Wang, W. P. Peng, H. C. Chang, N. Y. Hsu, W. B. Yang and C. H. Chen, 2007, “Matrix-assisted laser desorption/ionization (MALDI) mechanism revisited”, Analytica Chimica Acta, 582(1), 1-9. (SCIE)
  • N. Y. Hsu, W. B. Yang, C. H. Wong, Y. C. Lee, R. T. Lee, Y. S. Wang and C. H. Chen, 2007, “Matrix-assisted laser desorption/ionization mass spectrometry of polysaccharides with 2',4',6'-trihydroxyacetophenone as matrix”, Rapid Communications in Mass Spectrometry, 21, 2137-2146. (SCIE) (IF: 2.2; SCI ranking: 50%,59.7%,35.7%)
  •  C.-H. Wong, H.-Y. Hsu, K.-F. Hua, C.-H. Lin, J. Hsu, S.-T. Chen, K.-I. Lin, W.-B. Yang, J. Yu, A. Yu, 2007, “Methods and compositions associated with administration of an extract of Ganoderma lucidum”, European Patent Application, pp. No. 05810080.1.
  •  C.-H. Wong, H.-Y. Hsu, K.-F. Hua, C.-H. Lin, J. Hsu, S.-T. Chen, K.-I. Lin, W.-B. Yang, J. Yu, A. Yu, 2007, “Methods and compositions associated with administration of an extract of Ganoderma lucidum”, Japanese patent application, pp. No. 2007536927.
  • Y. M. Shao, W. B. Yang, H. P. Peng, M. F. Hsu, K. C. Tsai, T. H. Kuo, A. S. Yang, A. H. J. Wang, P. H. Liang, C. H. Lin and C. H. Wong, 2007, “Structure-Based Design and Synthesis of Highly Potent SARS-CoV 3CL Protease Inhibitors”, ChemBioChem, 8(14), 1654-1657. (SCIE)
  • H. J. Huang and W. B. Yang, 2007, “Synthesis of moenocinol and its analogs using BT-sulfone in Julia-Kocienski olefination”, Tetrahedron Lett, 48, 1429-1433. (SCIE)
  • A. Yu, J. Yu, K.-I. Lin, W.-B. Yang, C.-H. Wong, 2006, “Fungal immunostimulatory compositions”, Taiwan Patent Application, pp. No. 095137640.
  •  A. Yu, J. Yu, K.-I. Lin, W.-B. Yang, C.-H. Wong, 2006, “Fungal immunostimulatory compositions”, US Patent Application, pp. No. 11/549,215.
  • C.-H. Lin, W.-B. Yang, H.-C. Lin, 2006, “Glycosylation of exo-glycals”, US patent, pp. US 7,078,520 B2..
  •  C.-H. Wong, H.-Y. Hsu, K.-F. Hua, C.-H. Lin, J. Hsu, S.-T. Chen, K.-I. Lin, W.-B. Yang, J. Yu, A. Yu, 2006, “Methods and compositions associated with administration of an extract of Ganoderma lucidum”, Taiwan patent application, pp. No. 095131055.
  • K. I. Lin, Y. Y. Kao, H. K. Kuo, W. B. Yang, A. Chou, H. H. Lin, A. L. Yu and C. H. Wong, 2006, “Reishi polysaccharides induce immunoglobulin production through the TLR4/TLR2-mediated induction of transcription factor Blimp-1”, J. Biol. Chem, 281, 24111-24123. (SCIE)
  • Y. S. Cheng, K. H. Lo, H. H. Hsu, Y. M. Shao, W. B. Yang, C. H. Lin and C. H. Wong, 2006, “Screening for HIV protease inhibitors by protection against activity-mediated cytotoxicity in Escherichia coli”, J Virol Methods, 137, 82-87. (SCIE)
  • J. J. Shie, J. M. Fang, C. J. Kuo, T. H. Kuo, P. H. Liang, H. J. Huang, W. B. Yang, C. H. Lin, J. L. Chen, Y. T. Wu and C. H. Wong, 2005, “Discovery of Potent Anilide Inhibitors Against the Severe Acute Respiratory Syndrome 3CL Protease”, J. Med. Chem, 48, 4469-4473. (SCIE)
  • Shie, J. J., Fang, J. M., Kuo, C. J., Kuo, T. H., Liang, P. H., Huang, H. J., Yang, W. B., Lin, C. H., Chen, J. L., Wu, Y. T. and Wong, C. H., 2005, “Discovery of Potent Anilide Inhibitors against the Severe Acute Respiratory Syndrome 3CL Protease.”, J Med Chem, 48(13), 4469-4473. (SCIE)
  • C. H. Lin, H. C. Lin and W. B. Yang, 2005, “exo-Glycal Chemistry: General Aspects and Synthetic Applications for Biochemical Use”, Current Topics in Medicinal Chemistry, 5, 1431-1457. (SCIE)
  •  C.-H. Wong, H.-Y. Hsu, K.-F. Hua, C.-H. Lin, J. Hsu, S.-T. Chen, K.-I. Lin, W.-B. Yang, J. Yu, A. Yu, 2005, “Methods and compositions associated with administration of an extract of Ganoderma lucidum”, PCT patent, pp. PCT/US2005/036961.
  •  C.-H. Wong, C.-H. Lin, C.-C. Lin, K.-H. Khoo, S.-T. Chen, Y.-Y. Wang, W.-B. Yang, 2005, “Preparation and composition analysis of Immuno-modulating of Ganoderma lucidum polysaccharides F3”, US patent, pp. AS Docket Number: 28A-931014-1E.
  • H. C. Lin, W. B. Yang, Y. F. Gu, C. Y. Chen, C. Y. Wu and C. H. Lin, 2003, “Stereoselective glycosylation of exo-glycals accelerated by Ferrier-type rearrangement”, Org Lett, 5, 1087-1089. (SCIE)
  • C. F. Chang, W. B. Yang, C. C. Chang and C. H. Lin, 2002, “Inter- and intramolecular alcohol additions to exo-glycals”, Tetrahedron Lett, 43, 6515-6519. (SCIE)
  • W. B. Yang, Y. Y. Yang, Y. F. Gu, S. H. Wang, C. C. Chang and C. H. Lin, 2002, “Stereochemistry in the synthesis and reaction of exo-glycals”, J Org Chem, 67, 3773-3782. (SCIE)
  • W. B. Yang, S. S. Patil, C. H. Tsai, C. H. Lin and J.M. Fang, 2002, “The synthesis of -gulose and -xylose from -gluconolactone”, Tetrahedron, 58, 253-259. (SCIE)
  • W. B. Yang, C. Y. Wu and C. H. Lin, 2001, “Design, Synthesis, and Evaluation of Conjugated C-Glycosides as Novel Glycosidase Inhibitors”, Abstr Pap Am Chem Soc, 221, 74-.
  • W. B. Yang, C. F. Chang, S. H. Wang, C. F. Teo and C. H. Lin, 2001, “Expeditious synthesis of C-glycosyl conjugated dienes and aldehydes from sugar lactones”, Tetrahedron Lett, 42, 4657-4660. (SCIE)
  • W. B. Yang, C.Y. Wu, C. C. Chang, S. H. Wang, C. F. Teo and C. H. Lin, 2001, “Facile synthesis of conjugated exo-glycals”, Tetrahedron Lett, 42, 6907-6910. (SCIE)
  • W. B. Yang, Y. Y. Yang, C. F. Teo and C. H. Lin, 2000, “Regioselective Deprotection of Tert-Butyldimethylsilyl Ethers by Boron Trichloride”, Synlett, 2000, 1634-1636. (SCIE)
  • W. B. Yang, C. H. Tsai and C. H. Lin, 2000, “The expeditious preparation and reactivity of some protected forms of gluconolactones”, Tetrahedron Lett, 41, 2569-2572. (SCIE)
  • G. H. Chen, J. L. Hsu, W. B. Yang and J. M. Fang, 1999, “Preparation of Chiral Phosphorus(V) Reagents and Their Uses with Borane in the Enantioselective Reduction of Ketones”, JOURNAL OF THE CHINESE CHEMICAL SOCIETY, 46(5), 797-810. (SCIE)
  • W. B. Yang, J. M. Fang, 1998, “Asymmetric Addition of Trimethylsilyl Cyanide to Benzaldehydes Catalyzed by Samarium(III) Chloride and Chiral Phosphorus(V) Reagents”, J Org Chem, 63(4), 1356-1359. (SCIE)
  • J.-H. Chen, C.-Y. Yang, C.-H. Wong, W.-B. Yang,, Unpublished, “Determination of binding targets of an extract of Ganoderma lucidum using phage display”, Patent Application, pp. AS Docket Number: 28T-950913.
  • Y.-J. Chen, J.-H. Chen, M.-Y. Liao, W.-B. Yang, K.-I. Lin, C.-H. Wong, Unpublished, “Proteomic profiles of immune cells induced by an extract of Ganoderma lucidum”, Patent application, pp. AS Docket Number: 28T-950912.