NCLscan: accurate identification of non-co-linear transcripts (fusion, trans-splicing and circular RNA) with a good balance between sensitivity and precision
EDUCATION AND POSITIONS HELD:
- B.S., Department of Computer Science, Soochow University, Taiwan, 1988-1992.
- Ph.D., Institute of Computer and Information Science, National Chiao Tung University, Taiwan, 1992-1998.
- Postdoctoral Fellow, Institute of Biomedical Sciences, Academia Sinica, Taiwan (military service), 1998-2003.
- Assistant Professor, Genomics Research Center, Academia Sinica, Taiwan, 2003-2007.
- Associate Professor, Genomics Research Center, Academia Sinica, Taiwan, 2007-2014.
- Professor, Genomics Research Center, Academia Sinica, Taiwan, 2014-present.
- Division director of the Physical and Computational Genomics division, Genomics Research Center, Academia Sinica, 2022 – present
- Project for Excellent Junior Research Investigators Award, Ministry of Science and Technology, 2014-2018.
- Pius XI Medal, the Pontifical Academy of Sciences, Vatican, 2012.
- Academia Sinica Research Award for Junior Research Investigators, 2007.
- Wu Ta-Yuo Memorial Award, National Science Council, 2007.
- Post-doctoral Research Award of National Health Research Institutes (NHRI, Taiwan), 2000.
- Academia Sinica Post-doctoral Fellowship, 1999-2000.
- Academic Paper Awards from the Image Processing and Pattern Recognition (IPPR) Society, 1998.
- Bioinformatics and Big Data Analysis (生物資訊/大數據分析)
- Comparative & Evolutionary Genomics/Transcriptomics (比較與演化基因體/轉錄體)
- Post-transcriptional Regulation (後轉錄調控)
- Genomic imprinting (基因印記)
- Primate Evolution (靈長類動物演化)
- Systems Biology (系統生物學)
The goal of our studies is to comprehensively probe transcriptome complexity across species (especially for human vs. other non-human primates). It has been recognized that several post-transcriptional events such as alternative splicing (including cis- and trans-splicing) and RNA editing can generate many different transcript isoforms from the same genes, increasing the complexity of transcriptome/proteome. Despite the small genetic differences, human and other primates exhibit fairly different phenotypes. Some diseases attracting attention extensively such as Alzheimer's and Parkinson's disease, autism spectrum disorders, hepatitis B/C, AIDS, and cancers either are specific to human or have different symptoms/severity in non-human primates. Therefore, comparative genomics/transcriptomics analyses between human and non-human primates may not only increase our understanding of human (or primate) evolution but also radiate some light on the molecular mechanisms underlying such differences between primates in disease susceptibility. Recently, the next-generation sequencing technologies have been demonstrated to have the tremendous power of both profiling coverage and quantitative accuracy in genomics and transcriptomics studies. Based on such technologies, we analyze transcriptome variations (diversity/divergence) at both the DNA and RNA levels and try to shed light on decryption of different “RNA codes” in primates. Our research directions include:
- identification of gene structures and post-transcriptionally co-linear/non-co-linear events (including trans-spiced and circular RNAs);
- functional and evolutionary analysis of post-transcriptional events;
- investigation of dynamic transcriptomes during neural differentiation of human/non-human primate embryonic stem cells;
- identification and analysis of genetic variations between human and non-human primates;
- analysis of impacts of varied biological factors on evolutionary rates; and
- annotation of crop genomes.
本實驗室是一堅強的大數據分析實驗室，包含，資訊、統計、與分生實驗三個部分。我們以生物的觀點，資訊人的思維，解決純生物的問題。大數據分析是一門整合了資訊科學、統計學、與生命科學的跨領域研究。我們藉由設計演算法來分析新世代定序(NGS)資料，以資訊邏輯與數學運算將data轉換成information，配合統計分析篩選targets，之後輔以分生實驗驗證，並進一步實驗探討功能與機制，真正將information轉換成knowledge。我們的研究流程: data→information→knowledge。本實驗室致力於基因體(DNA level)和轉錄體(RNA level)跨物種間比較與演化等生物相關研究課題，特別著重於探討靈長類動物在後轉錄修飾如多樣性切割(Alternative Splicing包含trans-splicing、circular RNA、以及其他non-coding RNA)、RNA editing等。應用: 找出影響神經發育或退化相關疾病因子，目前focus on autism spectrum disorders(ASD,自閉症) 。 歡迎有志於學術研究的同學加入我們，組成堅強的台灣團隊，共同『挑戰未知、超越自我、台灣發光、放射國際』。
- Te-Lun Mai, Chia-Ying Chen, Yu-Chen Chen, Tai-Wei Chiang, and Trees-Juen Chuang*, 2022, “Trans-genetic effects of circular RNA expression quantitative trait loci and potential causal mechanisms in autism”, MOLECULAR PSYCHIATRY, 27, 4695-4706. (SCIE)
- Yen-Ju Chen, Chia-Ying Chen, Te-Lun Mai, Chih-Fan Chuang, Yu-Chen Chen, Sachin Kumar Gupta, Laising Yen, Yi-Da Wang, and Trees-Juen Chuang*, 2020, “Genome-wide, integrative analysis of circular RNA dysregulation and the corresponding circular RNA-microRNA-mRNA regulatory axes in autism”, GENOME RESEARCH, 30(3), 375-391. (SCIE)
- Te-Lun Mai, Trees-Juen Chuang*, 2019, “A-to-I RNA editing contributes to the persistence of predicted damaging mutations in populations”, GENOME RESEARCH, 29(11), 1766-1776. (SCIE)
- Chia-Ying Chen and Chuang Trees-Juen*, 2019, “Comment on “A comprehensive overview and evaluation of circular RNA detection tools””, PLOS COMPUTATIONAL BIOLOGY, 15, e1006158. (SCIE)
- Lien-Szu Wu, Wei-Cheng Cheng, Chia-Ying Chen, Ming-Che Wu, Yi-Chi Wang, Yu-Hsiang Tseng, Trees-Juen Chuang*, C-K James Shen*, 2019, “Transcriptomopathies of Pre- and Post-Symptomatic Frontotemporal Dementia-like Mice with TDP-43 Depletion in Forebrain Neurons”, Acta Neuropathologica Communications, 7:50. (SCIE)
- Chuang Trees-Juen*; Chen, Yen-Ju; Chen, Chia-Ying; Mai, Te-Lun; Wang, Yi-Da; Yeh, Chung-Shu; Yang, Min-Yu; Hsiao, Yu-Ting; Chang, Tien-Hsien; Kuo, Tzu-Chien; Cho, Hsin-Hua; Shen, Chia-Ning; Kuo, Hung-Chih; Lu, Mei-Yeh; Chen, Yi-Hua; Hsieh, Shan-Chi; Chiang, Tai-Wei, 2018, “Integrative transcriptome sequencing reveals extensive alternative trans-splicing and cis-backsplicing in human cells”, NUCLEIC ACIDS RESEARCH, 46(7), 3671-3691. (SCIE)
- Trees-Juen Chuang*, Chan-Shuo Wu, Chia-Ying Chen, Li-Yang Hung, Tai-Wei Chiang, and Min-Yu Yang, 2016, “NCLscan: accurate identification of non-co-linear transcripts (fusion, trans-splicing, and circular RNA) with a good balance between sensitivity and precision”, NUCLEIC ACIDS RESEARCH, 44, e29. (Corresponding & first author; SCI impact factor: 10.162; Rank (BIOCHEMISTRY & MOLECULAR BIOLOGY): 14/286; Q1). (SCIE)
- Iju Chen, Chia-Ying Chen and Tree-Juen Chuang*, 2015, “Biogenesis, identification, and function of exonic circular RNAs”, WIREs RNA, 6(5), 563-79 (Corresponding author; SCI impact factor: 4.838; Rank (CELL BIOLOGY): 53/189; Q2) the top 5 most cited articles over the past two years in WIREs RNA.. (SCIE)
- Trees-Juen Chuang* and Feng-Chi Chen*, 2014, “DNA methylation is associated with an increased level of conservation at nondegenerate nucleotides in mammals”, Molecular Biology and Evolution, 31(2), 387-396 (impact factor: 10.217; ranking: 14/290: BIOCHEMISTRY & MOLECULAR BIOLOGY). (SCIE)
- Chan-Shuo Wu, Chun-Ying Yu, Ching-Yu Chuang, Michael Hsiao, Cheng-Fu Kao, Hung-Chih Kuo* and Trees-Juen Chuang*, 2014, “Integrative transcriptome sequencing identifies trans-splicing events with important roles in human embryonic stem cell pluripotency”, Genome Research, 24(1), 25-36 (impact factor: 10.101; ranking: 3/160: BIOTECHNOLOGY & APPLIED MICROBIOLOGY). (SCIE)
- Chun-Ying Yu, Hsiao-Jung Liu, Li-Yuan Hung, Hung-Chih Kuo* and Trees-Juen Chuang*, 2014, “Is an Observed Non-co-linear RNA Product Spliced in trans, in cis, or just in vitro?”, NUCLEIC ACIDS RESEARCH, 42(14), 9410-9423 (Corresponding & first author; SCI impact factor: 10.162; Rank (BIOCHEMISTRY & MOLECULAR BIOLOGY): 14/286; Q1). (SCIE)
- Yi-Ching Chen, Jen-Hao Cheng, Zing Tsung-Yeh Tsai, Huai-Kuang Tsai*, Trees-Juen Chuang*, 2013, “The Impact of Trans-Regulation on the Evolutionary Rates of Metazoan Proteins”, NUCLEIC ACIDS RESEARCH, 41(13), 6371-6380 (impact factor: 8.278; ranking: 27/290: BIOCHEMISTRY & MOLECULAR BIOLOGY). (SCIE)
- Trees-Juen Chuang*, Feng-Chi Chen*, Yen-Zho Chen, 2012, “Position-dependent correlations between the level of DNA methylation and the evolutionary rates of mammalian coding exons”, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 109(39), 15841-15846 (impact factor: 9.737; ranking: 4/54: MULTIDISCIPLINARY SCIENCES). (SCIE)
- Chen SC, Chuang TJ, Li WH, 2011, “The relationships among microRNA regulation, intrinsically disordered regions, and other indicators of protein evolutionary rate.”, MOLECULAR BIOLOGY AND EVOLUTION, 28(9), 2513-2520 (impact factor: 10.217; ranking: 14/290: BIOCHEMISTRY & MOLECULAR BIOLOGY). (SCIE)
- Feng-Chi Chen, Chueng-Jong Chen, Wen-Hsiung Li, and Trees-Juen Chuang*, 2010, “Gene family size conservation is a good indicator of evolutionary rates”, Molecular Biology and Evolution, 27(8), 1750-1758 (impact factor: 10.353; ranking: 14/290: BIOCHEMISTRY & MOLECULAR BIOLOGY). (SCIE)
- Feng-Chi Chen, Yen-Zho Chen, and Trees-Juen Chuang*, 2009, “CNVVdb: a database of copy number variations across vertebrate genomes”, Bioinformatics, 25, 1419-1421. (SCIE)
- Yao-Ting Huang, Feng-Chi Chen*, Chiuan-Jung Chen, Hsin-Liang Chen and Trees-Juen Chuang*, 2008, “Identification and analysis of ancestral hominoid transcriptome inferred from cross-species transcript and processed pseudogene comparisons”, Genome Research, 18(7), 1163-1170 (impact factor: 14.397; ranking: 3/160: BIOTECHNOLOGY & APPLIED MICROBIOLOGY). (SCIE)
- Feng-Chi Chen, Shu-Miaw Chaw, Yun-Huei Tzeng, Sheng-Shun Wang, and Trees-Juen Chuang*, 2007, “Opposite Evolutionary Effects between Different Alternative Splicing Patterns”, Molecular Biology and Evolution, 24, 1443-1446 (impact factor: 10.353; ranking: 14/290: BIOCHEMISTRY & MOLECULAR BIOLOGY). (SCIE)
- Feng-Chi Chen, Sheng-Shun Wang, Shu-Miaw Chaw, Yao-Ting Huang and Trees-Juen Chuang*, 2007, “PGAA: a plant genome annotation pipeline for rice gene and alternatively spliced variant identification with cross-species EST conservationfrom seven plant species”, Plant Physiology, 143(3), 1086-1095 (impact factor: 6.555; ranking: 8/195: PLANT SCIENCES). (SCIE)
- Feng-Chi Chen, Chueng-Jong Chen, Wen-Hsiung Li* and Trees-Juen Chuang*, 2007, “Human-specific insertions and deletions inferred from mammalian genome sequences”, Genome Research, 17(1), 16-22 (impact factor: 14.397; ranking: 3/160: BIOTECHNOLOGY & APPLIED MICROBIOLOGY). (SCIE)
- Feng-Chi, Chen; Chueng-Jong, Chen; Trees-Juen, Chuang*, 2007, “INDELSCAN: a web server for comparative identification of species-specific and non-species-specific insertion/deletion events”, Nucleic Acids Research, 35(Web Server issue), W633-8 (impact factor: 8.278; ranking: 27/290: BIOCHEMISTRY & MOLECULAR BIOLOGY). (SCIE)
- F. C. Chen, S. S. Wang, C. J. Chen, W. H. Li and T. J. Chuang*, 2006, “Alternatively and Constitutively Spliced Exons Are Subject to Different Evolutionary Forces”, Mol Biol Evol, 23, 675-682 (impact factor: 10.353; ranking: 14/290: BIOCHEMISTRY & MOLECULAR BIOLOGY). (SCIE)
- F. C. Chen and T. J. Chuang*, 2006, “The effects of multiple features of alternatively spliced exons on the Ka/Ks ratio test”, BMC Bioinformatics, 7, 259. (SCIE)
- T. J. Chuang*, F. C. Chen and M. Y. Chou, 2004, “A comparative method for identification of gene structures and alternatively spliced variants”, Bioinformatics, 20, 3064-3079. (SCIE)
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