Chang, Chi-Fon

CFChang6x7Senior Research Specialist/Scientist




  • Ph.D. , Biophysics, The Ohio State University ,1997
  • Postdoctoral Fellow, Institute of Biomedical Sciences, Academia Sinica , 1997-1999
  • NMR Supporting Team Manager, Institute of Biomedical Sciences, Academia Sinica,1999-2002
  • NMR Facility Manager, High-Field Biomacromolecular Solution NMR Core Facility, Academia Sinica ,2002-present
  • Associate Research Specialist/Scientist, Genomics Research Center, Academia Sinica, 2004-2010
  • Senior Research Specialist/Scientist, Genomics Research Center, Academia Sinica, 2010-present


As a research specialist, I am responsible for the daily operation on NMR facility and assist researchers carrying out NMR related researches. I have dedicated myself to provide competent and efficient routine service, as well as all aspects of advance technical support on NMR spectroscopy. The duty of my job includes: keeping track of machine time usage; supervising the facility staff members; training users on using NMR spectrometers; developing and implementing new NMR experiments; assisting users setting up advanced NMR experiments; advising users on specific NMR experiments and applications; and providing technical consultation. My expertise is using biophysical methods, especially the NMR approach, to obtain structural and functional information for biomolecules.

 NMR Facility
 GRC NMR Facility


  • Lin MH, Cho CC, Chiu YC, Chien CY, Huang YP, Chang CF, Hsu CH, 2021, “Elucidating the tunability of binding behavior for the MERS-CoV macro domain with NAD metabolites.”, Communications biology, 4(1), 123. (SCIE)
  • Lin MH, Huang YP, Chang CF, Hsu CH, 2021, “NMR assignments of the macro domain from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).”, Biomolecular NMR assignments, 24, 1-6. (SCIE)
  • Mariasina Sofia S., Chang Chi‐Fon, Petrova Olga A., Efimov Sergey V., Klochkov Vladimir V., Kechko Olga I., Mitkevich Vladimir A., Sergiev Petr V., Dontsova Olga A., Polshakov Vladimir I., 2020, “Williams–Beuren syndrome‐related methyltransferase WBSCR27: cofactor binding and cleavage”, The FEBS Journal, DOI: 10.1111/febs.15320. (SCIE)
  • Mariasina SS, Petrova OA, Osterman IA, Sergeeva OV, Efimov SV, Klochkov VV, Sergiev PV, Dontsova OA, Huang TH, Chang CF*, Polshakov VI*, 2018, “NMR assignments of the WBSCR27 protein related to Williams-Beuren syndrome.”, Biomolecular NMR assignments, 12(2), 303-308. (SCIE)
  • Lin Tien-Wei, Chang Chi-Fon, Chang Yu-Jen, Liao Yi-Hung, Yu Hui-Ming, Chen Yun-Ru, 2017, “Alzheimer’s amyloid-β A2T variant and its N-terminal peptides inhibit amyloid-β fibrillization and rescue the induced cytotoxicity”, PLOS ONE, 12(3), e0174561. (SCIE)
  • Chou CY, Chu M, Chang CF, Yu T, Huang TH, Sakellariou D, 2016, “High sensitivity high-resolution full range relaxometry using a fast mechanical sample shuttling device and a cryo-probe.”, Journal of biomolecular NMR, 66(3), 187-194. (SCIE)
  • Huang YP, Cho CC, Chang CF*, Hsu CH*, 2016, “NMR assignments of the macro domain from Middle East respiratory syndrome coronavirus (MERS-CoV).”, Biomolecular NMR assignments, 10(2), 245-248. (SCIE)
  • Sabater Laurent, Fang Pei-Ju, Chang Chi-Fon, De Rache Aurore, Prado Enora, Dejeu Jérôme, Garofalo Antonio, Lin Jung-Hsin, Mergny Jean-Louis, Defrancq Eric, Pratviel Geneviève, 2015, “Cobalt(iii)porphyrin to target G-quadruplex DNA”, Dalton Transactions, 44(8), 3701-3707. (SCIE)
  • Hsu CH, Chang CF, Liao YD, Wu SH, Chen C, 2015, “Solution structure and base specificity of cytotoxic RC-RNase 2 from Rana catesbeiana.”, Archives of biochemistry and biophysics, 584, 70-8. (SCIE)
  • Huang SY, Chang CF, Fang PJ, Naik MT, Guntert P, Shih HM, Huang TH*, 2015, “The RING domain of human promyelocytic leukemia protein (PML).”, Journal of biomolecular NMR, 61(2), 173-80. (SCIE)
  • Li YC, Chang CK, Chang CF, Cheng YH, Fang PJ, Yu T, Chen SC, Li YC, Hsiao CD, Huang TH, 2014, “Structural dynamics of the two-component response regulator RstA in recognition of promoter DNA element.”, Nucleic acids research, 42(13), 8777-88. (SCIE)
  • Huang SY, Naik MT, Chang CF, Fang PJ, Wang YH, Shih HM, Huang TH, 2014, “The B-box 1 dimer of human promyelocytic leukemia protein.”, Journal of biomolecular NMR, 60(4), 275-81. (SCIE)
  • Ming-Yuan Su, Chung-I Chang and Chi-Fon Chang* , 2013, “1H, 13C and 15N resonance assignments of the pyrin domain from human PYNOD ”, Biomolecular NMR Assignments, 7(2), 141-143. (SCIE)
  • Ming-Yuan Su, Chiao-I Kuo, Chi-Fon Chang*, Chung-I Chang*, 2013, “Three-Dimensional Structure of Human NLRP10/PYNOD Pyrin Domain Reveals a Homotypic Interaction Site Distinct from Its Mouse Homologue”, PLoS One, 8(7), e67843. (SCIE)
  • Ariesandi W, Chang CF, Chen TE, Chen YR*, 2013, “Temperature-dependent structural changes of Parkinson\'s alpha-synuclein reveal the role of pre-existing oligomers in alpha-synuclein fibrillization.”, PloS one, 8(1), e53487. (SCIE)