研究副技師

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EDUCATION AND POSITIONS HELD:

• Ph.D., Department of Chemistry, Florida State University, 1989-1996
• Postdoctoral Research Fellow, Florida State University, 1996–1999
• Research Associate, Sinon Corporation, 1999-2000
• Postdoctoral Research Fellow, Institute of Biological Chemistry, Academia Sinica, 2000–2006
• Assistant Research Specialist, Genomics Research Center, Academia Sinica, 2006-2016
• Associate Research Specialist, Genomics Research Center, Academia Sinica, 2016-2024

FACILITY MANAGEMENT / EXPERTISE:

My laboratory works are mainly focusing on the synthesis of biologically active oligosaccharides to support GRC glycan array research programs. A long term goal is to establish a representative glycan library with sufficiently structural complexity and diversity to meet the demand. At present, I am maintaining a small but steadily growing collection of carbohydrate antigens to support glycan array development, such as early diagnosis of infectious diseases and cancers. I also collaborate with researchers in the area of carbohydrate-based vaccine development and glycopeptides synthesis.

主要的研究工作在於合成各種具有生物活性的寡醣分子，來支援基因體中心有關醣晶片的研究課題。長期目標是建立一個在分子結構上有足夠複雜性與差異性，具代表性的寡醣分子儲存庫，以符合研究上的需求。目前我負責維持一個小型但穩定成長的醣抗元分子庫，參與支援醣晶片應用在早期診斷癌症與傳染病方面的研究。同時也參與研究人員在發展醣分子疫苗的相關研究，以及支援醣蛋白方面的合成。

SELECTED PUBLICATIONS:

• Lin Tzu-Lung, Yang Feng-Ling, Ren Chien-Tai, Pan Yi-Jiun, Liao Kuo-Shiang, Tu I-Fan, Chang Yu-Pei, Cheng Yang-Yu, Wu Chung-Yi*, Wu Shih-Hsiung*, Wang Jin-Town*, 2022, “Development of Klebsiella pneumoniae Capsule Polysaccharide-Conjugated Vaccine Candidates Using Phage Depolymerases”, Frontiers in Immunology, 13, 843183, 1-13. (SCIE)
• Chen Chiang-Yun, Lin Yu-Wei, Wang Szu-Wen, Lin Yung-Chu, Cheng Yang-Yu, Ren Chien-Tai, Wong Chi-Huey, Wu Chung-Yi*, 2022, “Synthesis of Azido-Globo H Analogs for Immunogenicity Evaluation”, ACS Central Science, 8(1), 77-85. (SCIE)
• Chein-Hung Chen, Ya-Ping Lin, Chien-Tai Ren, Sachin S. Shivatare, Nan-Hon Lin, Chung-Yi Wu, Chung-Hsuan Chen, Jung-Lee Lin∗, 2020, “Enhancement of fucosylated N -glycan isomer separation with an ultrahigh column temperature in porous graphitic carbon liquid chromatography-mass spectrometry”, Journal of Chromatography A, 1632, 461610. (SCIE)
• Sujeet Pawar, Li Hsu, Thatikonda Narendar Reddy, Mettu Ravinder, Chien-Tai Ren, Yu-Wei Lin, Yang-Yu Cheng, Tzu-Wen Lin, Tsui-Ling Hsu, Sheng-Kai Wang, Chi-Huey Wong,* and Chung-Yi Wu*, 2020, “Synthesis of Asymmetric N‑Glycans as Common Core Substrates for Structural Diversification through Selective Enzymatic Glycosylation”, ACS CHEMICAL BIOLOGY, 15(9), 2382−2394. (SCIE)
• Lee I-Ming, Yang Feng-Ling, Chen Te-Li, Liao Kuo-Shiang, Ren Chien-Tai, Lin Nien-Tsung, Chang Yu-Pei, Wu Chung-Yi*, Wu Shih-Hsiung*, 2018, “Pseudaminic acid on exopolysaccharide of Acinetobacter baumannii plays a critical role in phage-assisted preparation of glycoconjugate vaccine with high antigenicity”, Journal of the American Chemical Society, 140(28), 8639-8643. (SCIE)
• Yasuhiko Kizuka, Miyako Nakano, Yoshiki Yamaguchi, Kazuki Nakajima, Ritsuko Oka, Keiko Sato, Chien-Tai Ren, Tsui-Ling Hsu, Chi-Huey Wong, and Naoyuki Taniguchi*, 2017, “An Alkynyl-Fucose Halts Hepatoma Cell Migration and Invasion by Inhibiting GDP-Fucose-Synthesizing Enzyme FX, TSTA3”, Cell Chemical Biology, 24, 1467–1478. (SCIE)
• I-Ming Lee, I-Fan Tu, Feng-Ling Yang, Tzu-Ping Ko, Jiahn-Haur Liao, Nien-Tsung Lin, Chung-Yi Wu, Chien-Tai Ren, Andrew H.-J. Wang, Ching-Ming Chang, Kai-Fa Huang*, Shih-Hsiung Wu*, 2017, “Structural basis for fragmenting the exopolysaccharide of Acinetobacter baumannii by bacteriophage ΦAB6 tailspike protein”, Scientific Reports, 7, 42711. (SCIE)
• • Sachin S Shivatare, Shih-Huang Chang, Tsung-I Tsai, Susan Yu Tseng, Yih-Shyan Lin,Yang-Yu Cheng, Chien-Tai Ren, Chang-Chun David Lee, Sujeet Pawar, Charng-Sheng Tsai,Hao-Wei Shih, Yi-Fang Zeng, Chi-Hui Liang Peter D. Kwong, Dennis R. Burton, Chung-Yi Wu,* Chi-Huey Wong*, 2016, “Modular synthesis of N-glycans and arrays for the hetero-ligand binding analysis of HIV antibodies”, Nature Chemistry, 8, 338-346. (SCIE)
• *Chen CH; Lin YP; Lin JL; Li ST; Ren CT; Wu CY; Chen CH., 2015, “Rapid identification of terminal sialic acid linkage isomers by pseudo-MS3 mass spectrometry”, ISRAEL JOURNAL OF CHEMISTRY, 55, 412-422. (SCIE)
• Hsin-Yu Lee, Chien-Yu Chen, Tsung-I Tsai, Shiou-Ting Li, Kun-Hsien Lin, Yang-Yu Cheng, Chien-Tai Ren, Ting-Jen R. Cheng, Chung-Yi Wu,* and Chi-Huey Wong*, 2014, “Immunogenicity Study of Globo H Analogues with Modification at the Reducing or Nonreducing End of the Tumor Antigen”, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 136(48), 16844−16853.. (SCIE)
• Sachin S. Shivatare, Shih-Huang Chang, Tsung-I Tsai,Chien-Tai Ren, Hong-Yang Chuang,Li Hsu, Chih-Wei Lin, Shiou-Ting Li, Chung-Yi Wu,*and Chi-Huey Wong*, 2013, “Efficient Convergent Synthesis of Bi‑, Tri‑, and Tetra-antennary Complex Type N‑Glycans and Their HIV‑1 Antigenicity”, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 135, 15382−15391. (SCIE)
• Hong-Yang Chuang, Chien-Tai Ren, Chung-An Chao, Chung-Yi Wu, Sachin S Shivatare, Ting-Jen R. Cheng,Chung-Yi Wu*, and Chi-Huey Wong*, 2013, “Synthesis and Vaccine Evaluation of the Tumor Associated Carbohydrate Antigen RM2 from Prostate Cancer”, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 135, 11140-11150. (SCIE)
• Yen-Lin Huang, Jung-Tung Hung, Sarah K. C. Cheung, Hsin-Yu Lee, Kuo-Ching Chu, Shiou-Ting Li, Yu-Chen Lin, Chien-Tai Ren, Ting-Jen R. Cheng, Tsui-Ling Hsu, Alice L. Yu, Chung-Yi Wu*, and Chi-Huey Wong* , 2013, “Carbohydrate-based vaccines with a glycolipid adjuvant for breast cancer”, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 110, 2517-2522. (SCIE)
• Ting-Jen R. Cheng, Shi-Yun Wang, Wen-Hsien Wen, Ching-Yao Su, Mengi Lin, Wen-I Huang, Ming-Tsan Liu, Ho-Sheng Wu, Nung-Sen Wang, Chung-Kai Cheng, Chun-Lin Chen, Chien-Tai Ren, Chung-Yi Wu, Jim-Min Fang,* Yih-Shyun E. Cheng,* and Chi-Huey Wong*, 2013, “Chemical Probes for Drug-Resistance Assessment by Binding Competition (RABC): Oseltamivir Susceptibility Evaluation”, ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 52(1), 366-370. (SCIE)
• Chen-Shiou Wu, Chia-Jui Yen, Ruey-Hwang Chou, Shiou-Ting Li, Wei-Chien Huang, Chien-Tai Ren, Chung-Yi Wu*, Yung-Luen Yu*, 2012, “Cancer-Associated Carbohydrate Antigens as Potential Biomarkers for Hepatocellular Carcinoma”, PLoS One, 7(7), e39466. (SCIE)
• Chen, M. W.; Liao, H.-Y.; Huang, Y.; Jan, J.-T.; Huang, C.-C.; Ren, C.-T.; Wu, C.-Y.; Cheng, T.-J.; Ho, D.*; Wong, C.-H.*, 2011, “Broadly neutralizing DNA vaccine with specific mutation alters the antigenicity and sugar binding activities of influenza hemagglutinin”, Proceedings of the National Academy of Sciences of the United States of America, 108(9),3510-3515. (SCIE)
• Chu, K.-C.; Ren, C.-T.; Liu, C.-P.; Hsu, C.-H.; Sun, T.-H.; Han, J.-L.; Pal, B.; Chao, T.-A.; Lin, Y.-F.; Wu, S.-H.; Wong, C.-H.*; Wu, C.-Y.*, 2011, “Efficient and Stereoselective Synthesis of alpha(2→9) Oligosialic acids: From Monomers to Dodecamers”, Angewandte Chemie International Edition, 50, 9391-9395. (SCIE)
• Tsai, B.-L.; Han, J.-L.; Ren, C.-T.; Wu, C.-Y.*; Wong, C.-H., 2011, “Programmable One-Pot Synthesis of Tumor-Associated Carbohydrate Antigens Lewis X dimer and KH-1 epitopes”, Tetrahedron Letters, 52, 2132-2135. (SCIE)
• Liao, H.-Y., Hsu, C.-H., Wang, S.-C., Liang C.-H., Ye H.-Y., Su C.-Y., Jan J.-T., Ren C.-T., Cheng T.-J. R.,* Wu, C.-Y.*, Wong C.-H.*, 2010, “Differential Receptor Binding Affinities of Influenza Hemagglutinins on Glycan Arrays”, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 132, 14849-14856. (SCIE)
• Hsu, C.-H.; Chu, K.-C.; Lin, Y.-S.; Han, J.-L.; Peng, Y.-S.; Ren, C.-T.;Wu, C.-Y. and Wong, C.-H., 2010, “Highly Alpha-Selective Sialyl Phosphate Donors for Efficient Preparation of Natural Sialosides”, Chemistry-A European Journal, 16(6),1754-1760. (SCIE)
• Wang, S. Y.; Su, C. Y.; Lin, M.; Huang, S. Y.; Huang, W. I.; Wang, C. C.; Wu, Y. T.; Cheng, T. J. R.; Yu, H. M.; Ren, C. T.; Wu, C. Y.; Wong, C. H.; Cheng. Y. S. E., 2009, “HA-pseudotyped retroviral vectors for influenza antagonist screening”, JOURNAL OF BIOMOLECULAR SCREENING, 14, 294-302.
• Cheng-Chi Wang, Yen-Lin Huang, Chien-Tai Ren, Chin-Wei Lin, Jung-Tung Hung, Jyh-Cherng Yu, Alice L. Yu, Chung-Yi Wu, and Chi-Huey Wong , 2008, “Glycan microarray of Globo H and related structures for quantitative analysis of breast cancer”, Proceedings of the National Academy of Sciences of the United States of America, 105, 11661-11666. (SCIE)
• Nien-Yeen Hsu, Susan Yu Tseng, Chung-Yi Wu, Chien-Tai Ren, Yuan-Chang Lee, Chi-Huey Wong and Chung-Hsuan Chen , 2008, “Desorption ionization of biomolecules on metals”, ANALYTICAL CHEMISTRY, 80, 5203-5210. (SCIE)
• Lu, C.-P., Ren, C.-T., Wu, S.-H.*, Chu, C.-Y., Lo, L.-C.*, 2007, “Development of an activity-based probe for steroid sulfatases”, CHEMBIOCHEM, 8(18), 2187-2190. (SCIE)
• Chien-Tai Ren, Yu-Hsuan Tsai, Yu-Liang Yang, Wei Zou and Shih-Hsiung Wu, 2007, “Synthesis of a Tetrasaccharide Glycosyl Glycerol. Precursor to Glycolipids of Meiothermus taiwanensis ATCC-BAA-400”, J. Org. Chem, 72(14), 5427-5430. (SCIE)
• An-Tai Wu, Yi-Ping Yu, Chien-Tai Ren, Wei Zou and S.-H. Wu, 2005, “9-O-Sulfation on α-NeuAc-(2-8)-NeuAc and inter-residue lactonization”, CARBOHYDRATE RESEARCH, 340, 1219-1223. (SCIE)
• Chun-Ping Lu, Chien-Tai Ren, Yi-Ning Lai, S.-H. Wu, Wei-Man Wang, Jean-Yin Chen and Lee-Chiang Lo, 2005, “Design of a Mechanism-Based Probe for Neuraminidase to Capture Influenza Viruses”, ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 44, 6888-6892. (SCIE)
• Hao.-Ping Chen, Fang-Ciao Hsui, Li-Ying Lin, Chien-Tai Ren and S.-H. Wu, 2004, “Coexpression, purification and characterization of the E and S subunits of coenzyme B12 and B6 dependent Clostridium sticklandii D-ornithine aminomutase in Escherichia coli”, European Journal of Biochemistry, 271, 4293-4297.
• C.-T. Ren, C.-S. Chen, Y.-P. Yu, Y.-F. Tsai, P.-Y. Lin, Y.-J. Chen, W. Zou and S.-H. Wu, 2003, “Synthesis of α-(2→5)Neu5Gc Oligomers”, CHEMISTRY-A EUROPEAN JOURNAL, 9, 1085-1095. (SCIE)
• C.-T. Ren, C.-S. Chen and S.-H. Wu, 2002, “Synthesis of a Sialic Acid Dimer Derivative 2α-O-Benzyl Neu5Ac-α-(2-5) Neu5Gc”, J. Org. Chem, 67, 1376-1379. (SCIE)