研究技師

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chifon
02-27871236

 

EDUCATION AND POSITIONS HELD:

• Ph.D. , Biophysics, The Ohio State University ,1997
• Postdoctoral Fellow, Institute of Biomedical Sciences, Academia Sinica , 1997-1999
• NMR Supporting Team Manager, Institute of Biomedical Sciences, Academia Sinica,1999-2002
• NMR Facility Manager, High-Field Biomacromolecular Solution NMR Core Facility, Academia Sinica ,2002-present
• Associate Research Specialist/Scientist, Genomics Research Center, Academia Sinica, 2004-2010
• Senior Research Specialist/Scientist, Genomics Research Center, Academia Sinica, 2010-present

FACILITY MANAGEMENT / EXPERTISE:

As a research specialist, I am responsible for the daily operation on NMR facility and assist researchers carrying out NMR related researches. I have dedicated myself to provide competent and efficient routine service, as well as all aspects of advance technical support on NMR spectroscopy. The duty of my job includes: keeping track of machine time usage; supervising the facility staff members; training users on using NMR spectrometers; developing and implementing new NMR experiments; assisting users setting up advanced NMR experiments; advising users on specific NMR experiments and applications; and providing technical consultation. My expertise is using biophysical methods, especially the NMR approach, to obtain structural and functional information for biomolecules.

GRC NMR Facility

主要負責管理基因體中心核磁共振核心實驗室並參與或支援研究人員核磁共振相關研究。服務內容包括：管理核心工作小組確保核心軟硬體正常運作；調控核心所有儀器的時間分配；提供使用者技術及實驗規劃之諮詢；協助蒐集分析圖譜; 訓練新進使用者; 舉辦教育訓練課程；發展或引進核磁共振相關新技術；參與核磁共振相關研究計畫。

SELECTED PUBLICATIONS:

• Chang YC, Hsieh ML, Lee HL, Hee SW, Chang CF, Yen HY, Chen YA, Chen YR, Chou YW, Li FA, Ke YY, Chen SY, Hung MS, Hung AF, Huang JY, Chiu CH, Lin SY, Shih SF, Hsu CN, Hwang JJ, Yeh TK, Cheng TR, Liao KC, Laio D, Lin SW, Chen TY, Hu CM, Vogel U, Saar D, Kragelund BB, Tsou LK, Tseng YH, Chuang LM, 2025, “Identification of PTGR2 inhibitors as a new therapeutic strategy for diabetes and obesity.”, EMBO molecular medicine, 17(5), 938-966. (SCIE)
• Lin GM, Yu TA, Chang CF, Hsu CH, 2024, “Proline Isomerization and Molten Globular Property of TgPDCD5 Secreted from Toxoplasma gondii Confers Its Regulation of Heparin Sulfate Binding.”, JACS Au, 4(5), 1763-1774. (SCIE, SSCI, A&HCI, Scopus, EI, TSSCI, THCI, Others)
• Mariasina Sofia S., Chang Chi-Fon, Navalayeu Tsimafei L., Chugunova Anastasia A., Efimov Sergey V., Zgoda Viktor G., Ivlev Vasily A., Dontsova Olga A., Sergiev Petr V., Polshakov Vladimir I.*, 2022, “Williams-Beuren Syndrome Related Methyltransferase WBSCR27: From Structure to Possible Function”, Frontiers in Molecular Biosciences, 9, 865743. (SCIE)
• Lin MH, Huang YP, Chang CF*, Hsu CH*, 2021, “NMR assignments of the macro domain from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).”, Biomolecular NMR assignments, 15(1), 137-142. (SCIE)
• Lin MH, Cho CC, Chiu YC, Chien CY, Huang YP, Chang CF, Hsu CH, 2021, “Elucidating the tunability of binding behavior for the MERS-CoV macro domain with NAD metabolites.”, Communications biology, 4(1), 123. (SCIE)
• Mariasina SS, Chang CF, Petrova OA, Efimov SV, Klochkov VV, Kechko OI, Mitkevich VA, Sergiev PV, Dontsova OA, Polshakov VI, 2020, “Williams-Beuren syndrome-related methyltransferase WBSCR27: cofactor binding and cleavage.”, The FEBS journal, 287(24), 5375-5393. (SCIE)
• Mariasina SS, Petrova OA, Osterman IA, Sergeeva OV, Efimov SV, Klochkov VV, Sergiev PV, Dontsova OA, Huang TH, Chang CF*, Polshakov VI*, 2018, “NMR assignments of the WBSCR27 protein related to Williams-Beuren syndrome.”, Biomolecular NMR assignments, 12(2), 303-308. (SCIE)
• Chou CY, Chu M, Chang CF, Yu T, Huang TH, Sakellariou D, 2016, “High sensitivity high-resolution full range relaxometry using a fast mechanical sample shuttling device and a cryo-probe.”, Journal of biomolecular NMR, 66(3), 187-194. (SCIE)
• Huang YP, Cho CC, Chang CF*, Hsu CH*, 2016, “NMR assignments of the macro domain from Middle East respiratory syndrome coronavirus (MERS-CoV).”, Biomolecular NMR assignments, 10(2), 245-248. (SCIE)
• Hsu CH, Chang CF, Liao YD, Wu SH, Chen C, 2015, “Solution structure and base specificity of cytotoxic RC-RNase 2 from Rana catesbeiana.”, Archives of biochemistry and biophysics, 584, 70-8. (SCIE)
• Ming-Yuan Su, Chung-I Chang and Chi-Fon Chang* , 2013, “1H, 13C and 15N resonance assignments of the pyrin domain from human PYNOD ”, Biomolecular NMR Assignments, 7(2), 141-143. (SCIE)
• Ming-Yuan Su, Chiao-I Kuo, Chi-Fon Chang*, Chung-I Chang*, 2013, “Three-Dimensional Structure of Human NLRP10/PYNOD Pyrin Domain Reveals a Homotypic Interaction Site Distinct from Its Mouse Homologue”, PLoS One, 8(7), e67843. (SCIE)
• Chou CY, Chu M, Chang CF, Huang TH*, 2012, “A compact high-speed mechanical sample shuttle for field-dependent high-resolution solution NMR.”, Journal of magnetic resonance (San Diego, Calif. : 1997), 214(1), 302-8.