Wang, Yi Sheng

Researcher / Professor


wergateEmail
+886-2-27871272

 

 

EDUCATION AND POSITIONS HELD:

  • Ph.D., Chemistry, National Taiwan University, 2001
  • Postdoctoral Fellow, Institute of Atomic and Molecular Sciences (IAMS), Academia Sinica, 2001-2005
  • Postdoctoral Fellow, National High Magnetic Field Laboratory (NHMFL), Florida State University, U.S.A. 2002-2003
  • Assistant Professor, Genomics Research Center, Academia Sinica, 2005-2011
  • Associate Professor, Genomics Research Center, Academia Sinica, 2011-2019
  • Research Fellow, Genomics Research Center, Academia Sinica, 2019-present

HONORS:

  • Outstanding Students Conference Travel Grant, 2000
  • Advanced Abroad Research Fellowship, 2002-2003
  • Outstanding Post-Doctor Researchers Conference Travel Grant, 2002 & 2004

RESEARCH INTERESTS:

Biophysical Chemistry and Technology Development

Our objective is the advancement of bio-analytical sciences and biophysics in a systematic and complementary manner. Both fundamental biophysical knowledge and technical innovations are essential for resolving critical problems, such as high-throughput diagnoses, accurate quantifications and molecular function examinations. We have studied the physics and chemistries involved in the development of key analytical technologies, and integrated them for express clinical diagnoses. The fundamental study of ionization mechanism of biomolecules is one of our focuses, which enables us to design an effective strategy to improve the detection of carbohydrates. The technologies developed in this laboratory include:

  • Synchronized Dual-Polarity Mass Spectrometry
  • Synchronized Dual-Polarity Imaging Mass Spectrometry
  • Soft Protonation Method for Carbohydrates
  • Ultra-Sensitive Surface Ionization Method for Carbohydrates
  • Structural and Energetic Simulation of Oligosaccharides
  • Nanoparticles for Selective Purification of Phosphoproteins
  Dual-polarity imaging_mass
Dual-polarity imaging mass spectrometer and analytical results of insulin A-chain with matrix on surface. 

 

SELECTED PUBLICATIONS:

  • Wang Chia-Chen, Wang Yi-Sheng*, to appear, “Effective analysis of degree of polymerization of polysialic acids in mass spectrometry by combining novel sample preparation and dynamic instrument optimization methods”, Carbohydrate Research, 471, 78-84. (SCIE)
  • C.-H. Hsiao, Y.-H. Lai, S.-Y. Kuo, Y.-H. Cai, C.-H. Lin, Y.-S. Wang*, 2020, “A Dynamic Data Correction Method for Enhancing Resolving Power of Integrated Spectra in Spectroscopic Analysis”, ANALYTICAL CHEMISTRY, 92(19), 12763-12768. (SCIE)
  • Ou Yu-Meng, Kuo Shu-Yun, Lee Hsun, Chang Huan-Tsung, Wang Yi-Sheng*, 2018, “An Efficient Sample Preparation Method to Enhance Carbohydrate Ion Signals in Matrix-assisted Laser Desorption/Ionization Mass Spectrometry”, Jove-Journal of Visualized Experiments, 137, e57660. (SCIE)
  • Wei Shih-Chun, Fan Shen, Lien Chia-Wen, Unnikrishnan Binesh, Wang Yi-Sheng, Chu Han-Wei, Huang Chih-Ching, Hsu Pang-Hung, Chang Huan-Tsung*, 2018, “Graphene oxide membrane as an efficient extraction and ionization substrate for spray-mass spectrometric analysis of malachite green and its metabolite in fish samples”, Analytica Chimica Acta, 1003, 42-48. (SCIE)
  • Cai Yi-Hong, Wang Yi-Sheng*, 2018, “Impact of uneven sample morphology on mass resolving power in linear MALDI-TOF mass spectrometry: A comprehensive theoretical investigation”, Journal of Mass Spectrometry, 53(4), 361-368. (SCIE)
  • H. Lee, Y.-H. Lai, Y.-M. Ou, C.-W. Tsao, Y.-J. Jheng, S.-Y. Kuo, H.-T. Chang, Y.-S. Wang*, 2017, “Enhancing carbohydrate ion yield by controlling crystalline structures in matrix-assisted laser desorption/ionization mass spectrometry”, ANALYTICA CHIMICA ACTA, 994, 49-55. (SCIE)
  • C.-C. Wang, Y.-H. Lai, Y.-M. Ou, H.-T. Chang, Y.-S. Wang*, 2016, “Critical Factors Determining Quantification Capability of MALDI-TOF Mass Spectrometry”, PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY A-MATHEMATICAL PHYSICAL AND ENGINEERING SCIENCES, 374(2079), 20150371. (SCIE)
  • Y.-M. Ou, C.-W. Tsao, Y.-H. Lai, H. Lee, H.-T. Chang, Y.-S. Wang*, 2016, “Preparation of homogeneous MALDI samples for quantitative applications”, JOVE-Journal of Visualized experiments, 116, e54409. (SCIE)
  • Urban, P.; Chen, Y.-C.; Wang, Y.-S., 2016, Time-Resolved Mass Spectrometry, 320 pages, London: John Wiley & Sons, Ltd.
  • Primadona Indah, Lai Yin-Hung, Capangpangan Rey Y., Obena Rofeamor P., Tseng Mei-Chun, Huang Ming-Feng, Chang Huan-Tsung, Li Shiou-Ting, Wu Chung-Yi, Chien Wei-Ting, Lin Chun-Cheng, Wang Yi-Sheng,* Chen Yu-Ju*, 2016, “Functionalized HgTe nanoparticles promote laser-induced solid phase ionization/dissociation for comprehensive glycan sequencing”, The Analyst, 141(21), 6093-6103. (SCIE)
  • Dutkiewicz E. P., Hsieh K.-T., Wang Y.-S., Chiu H.-Y., Urban P. L., 2016, “Hydrogel Micropatch and Mass Spectrometry-Assisted Screening for Psoriasis-Related Skin Metabolites”, Clinical Chemistry, 62(8), 1120-1128. (SCIE)
  • Chang, K.-K.; Cai, Y.-H.; Chen, C.-H.; Wang, Y.-S.;*, 2016, “Neutralization charge detection method for detecting ions under ambient and liquid phases.”, CHEMICAL COMMUNICATIONS, 52(29), 5187-5189. (SCIE)
  • Y.-H. Lai, Y.-H. Cai, H. Lee, Y.-M. Ou, C.-H. Hsiao, C.-W. Tsao, H.-T. Chang, Y.-S. Wang*, 2016, “Reducing spatial heterogeneity of MALDI samples with Marangoni flows during sample preparation”, JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 27(8), 1314-1321. (SCIE)
  • Cai, Y.-H.; Lai, Y.-H.; Wang, Y.-S.*, 2015, “Coupled space- and velocity-focusing in time-of-flight mass spectrometry – a comprehensive theoretical investigation.”, JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 26(10), 1722-1731. (SCIE)
  • 台灣質譜學會,2014,《質譜分析技術原理與應用》,共800頁,台北市:全華圖書。
  • Ozdemir, A.; Lin, J.-L.; Wang, Y.-S.; Chen, C.-H.*, 2014, “A deeper look into sonic spray ionization.”, RSC ADVANCES, 4(106), 61290-61297. (SCIE)
  • Lai, Y.-H.; Chen, B.-G.; Lee, Y. T.; Wang, Y.-S.;* Lin, S. H.*, 2014, “Contribution of thermal energy to initial ion production of matrix-assisted laser desorption/ionization observed with 2,4,6-trihydroxyacetophenone.”, RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1716-1722. (SCIE) (IF: 2.2; SCI ranking: 50%,59.7%,35.7%)
  • Dutkiewicz, E.; Lin, J.-D.; Tseng, T.-W.; Wang, Y.-S.; Urban, P.*, 2014, “Hydrogel micropatches for sampling and profiling skin metabolome.”, ANALYTICAL CHEMISTRY, 86 (5), 2337-2344. (SCIE)
  • C.-L. Wu, C.-C. Wang, Y.-H. Lai, H. Lee, J.-D. Lin, Y. T. Lee, Y.-S. Wang*, 2013, “Selective enhancement of carbohydrate ion abundances by diamond nanoparticles for mass spectrometric analysis.”, ANALYTICAL CHEMISTRY, 85, 3836-3841. (SCIE)
  • Y. Y. Zeng, H.-J. Chen, K. J. Shiau, S.-U. Hung, Y.-S. Wang, C.-C. Wu, 2012, “Efficient enrichment of phosphopeptides by magnetic TiO2-coated carbon-encapsulated iron nanoparticles ”, PROTEOMICS, 12 (3), 380-390. (SCIE)
  • O. P. Charkin, N. M. Klimenko, Y.-S. Wang, 2012, HANDBOOK ON FULLERENE: SYNTHESIS, PROPERTIES AND APPLICATIONS, 313-334 pages, USA: Nova Science Publishers, Inc..
  • Y.-H. Lai, C.-C. Wang, B.-H. Liu, C. W. Chen, S.-H. Lin, Y. T. Lee, Y.-S. Wang*, 2012, “Analysis of initial reactions of MALDI based on chemical properties of matrices and excitation condition.”, JOURNAL OF PHYSICAL CHEMISTRY B, 116(32), 9635-9643. (SCIE)
  • O. P. Charkin,* N. M. Klimenko, Y.-S. Wang, 2012, “Theoretical Study of C60(OH)20 and C60(OH)18 fullerenols and B12(OH)122-, B12(OH)122-, and B12(OH)122- polyhydroxyl clusters and their Li-substituted derivatives.”, RUSSIAN JOURNAL OF INORGANIC CHEMISTRY, 57(7), 970-980. (SCIE)
  • C.-H. Hsiao, C.-W. Hong, B.-H. Liu, C. W. Chen, C.-C. Wu, Y.-S. Wang, 2011, “Comprehensive Molecular Imaging of Photolabile Surface Samples with Synchronized Dual-Polarity Time-of-Flight Mass Spectrometry”, RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 25(7), 834-842. (SCIE) (IF: 2.2; SCI ranking: 50%,59.7%,35.7%)
  • O. P. Charkin, N. M. Klimenko, Y.-S. Wang, C.-C. Wang, C.-H. Chen, S.-H. Lin, 2011, “Theoretical and experimental study of fullerenol molecules and ion C60(OH)24-n(OL)n and C60(OH)24-n(OL)nL+, substituted consecutively by alkali metal atoms L (n = 1 - 24)”, RUSSIAN JOURNAL OF INORGANIC CHEMISTRY, 56(4), 580-590. (SCIE)
  • Y.-H. Lai, C.-C. Wang, S.-H. Lin, Y. T. Lee, Y.-S. Wang*, 2010, “Solid-Phase Thermodynamic Interpretation of Ion Desorption in Matrix-Assisted Laser Desorption/Ionization”, JOURNAL OF PHYSICAL CHEMISTRY B, 114 (43), 13847–13852. (SCIE)
  • B.-H. Liu, O. P. Charkin, N. Klemenko, C. W. Chen, Y.-S. Wang*, 2010, “Initial ionization reaction in matrix-assisted laser/desorption ionization”, JOURNAL OF PHYSICAL CHEMISTRY B, 114, 10853-10859. (SCIE)
  • Liang, C.-W.; Lee, Y. T.; Chen, C.-H.; Wang, Y.-S., 2010, “Ionizing nonvolatile samples using laser desorption-proton-transfer reaction with cluster reagent ions”, International Journal of Mass Spectrometry, 291 (1-2), 61-66. (SCIE)
  • Chen, H.-K.; Chang, C.-K.; Wu, C.-C.; Huang, M.-C.; Wang, Y.-S., 2009, “Synchronized dual-polarity electrospray ionization mass spectrometry”, JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 20 (12), 2254-2257.. (SCIE)
  • Bo-Hong Liu, Yuan Tseh Lee, Yi-Sheng Wang, 2009, “Incoherent production reactions of positive and negative ions in matrix-assisted laser desorption/ionization”, Journal of the American Society for Mass Spectrometry, 20(6), 1078-1086.. (SCIE)
  • Wang, Y.-S.; Chen H.-K.; Huang M.-C.; Wu, C.-C., 2008, “Synchronized dual-polarity ion production using electrospray ionization method”, paper presented at The 56th ASMS Conference on Mass Spectrometry and Allied Topics, Colorado, U.S.A.: American Society for Mass Spectrometry, 2008-06-01 ~ 2008-06-05.
  • Chang C.-K.;Wu, C.-C.;* Wang, Y.-S.; Chang, H.-C., 2008, “Selective extraction and enrichment of multiphosphorylated peptides using polyarginine-coated diamond nanoparticles”, Analytical Chemistry, 80(10), 3791-3797. (SCIE)
  • Tsai, S.-T.; Chen, C.-H.; Lee, Y. T.; Wang, Y.-S.,*, 2008, “Desorption dynamics of neutral molecules in matrix-assisted laser desorption/ionization.”, Molecular Physics, 106 (1-2), 239-247. (SCIE)
  • Jessome, L.; Hsu, N.-Y.; Wang, Y.-S.; Chen, C.-H.,*, 2008, “Matrix-assisted laser desorption/ionization mechanism study with dihydroxybenzoic acid isomers as matrices”, Rapid Communications in Mass Spectrometry, 22(2), 130-134.. (SCIE) (IF: 2.2; SCI ranking: 50%,59.7%,35.7%)
  • Li, M.-H.; Tsai, S.-T.; Chen, C.-H.; Wang, Y.-S., 2007, “Bipolar ion detector with enhanced sensitivity by sequential conversion reactions”, TP039 pages, paper presented at The 55th ASMS Conference on Mass Spectrometry and Allied Topics, Indiana, USA: American Society for Mass Spectrometry, 2007-06-03 ~ 2007-06-07.
  • Wang, Y.-S.; Li, M.-H.; Chen, C.-H.; Lee, Y. T., 2007, “Pressure-insensitive bipolar ion detector (BID) based on sequential conversion reaction”, WOFpm0350 pages, paper presented at The 55th ASMS Conference on Mass Spectrometry and Allied Topics, USA: American Society for Mass Spectrometry, 2007-06-03 ~ 2007-06-07.
  • M.-H. Li, S.-T. Tsai, C.-H. Chen, C. W. Chen, Y. T. Lee and Y.-S. Wang, 2007, “Bipolar Ion Detector Based on Sequential Conversion Reactions”, Analytical Chemistry, 79 (4), 1277-1282.. (SCIE)
  • W. C. Chang, L. C. L. Huang, Y.-S. Wang, W.-P. Peng, H.-C. Chang, N. Y. Hsu, W. B. Yang and C. H. Chen, 2007, “Matrix-assisted laser desorption/ionization (MALDI) mechanism revisited”, Analytica Chimica Acta, 582, 1-9. (SCIE)
  • N. Y. Hsu, W. B. Yang, C. H. Wong, Y. C. Lee, R. T. Lee, Y. S. Wang and C. H. Chen, 2007, “Matrix-assisted laser desorption/ionization mass spectrometry of polysaccharides with 2',4',6'-trihydroxyacetophenone as matrix”, RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 21, 2137-2146. (SCIE) (IF: 2.2; SCI ranking: 50%,59.7%,35.7%)
  • S. Sabu, F. C. Yang, Y. S. Wang, W. H. Chen, M. I. Chou, H. C. Chang and C. C. Han, 2007, “Peptide analysis: Solid phase extraction-elution on diamond combined with atmospheric pressure matrix-assisted laser desorption/ionization-Fourier transform ion cyclotron resonance mass spectrometry”, Anal Biochem, 367, 190-200. (SCIE)
  • Huang L.-C.; Wang Y.-S.; Chen C.-H., 2006, “Microchannel plate detection efficiencies of large biomolecules”, paper presented at The 54th American Society for Mass Spectrometry conference on Mass Spectrometry and Allied Topics, Washington, U.S.A.: American Society for Mass Spectrometry, 2006-05-28 ~ 2006-06-01.
  • Y.-S. Wang, S.-C. Wei, C.-C. Kau, S. Sahadevan, Y. T. Lee, C.-C. Han, H.-C. Chang and Z. H. J. Zhang, 2006, “Dissociation of heme from gaseous myoglobin ions: A combined study with FTICR mass spectrometry, IRMPD spectroscopy and DFT calculations”, Journal of Chemical Physics, 125 (13), 133310. (SCIE)
  • Huang, L. C. L.; Chen, C.-H.; Wang, Y.-S., 2006, “Investigating production reactions of positive and negative ions in MALDI”, P-51 pages, paper presented at Trends in Chemical Dynamics: From Small Molecules to Biomolecules, Taiwan, R.O.C.: Academia Sinica, 2006.
  • Hsu, N.-Y.; Yang, W.-B.; Wong, C.-H.; Lee, Y.-C.; Wang, Y.-S.; Chen, C.-H., 2006, “MALDI of polysaccharide with THAP as matrix”, P-50 pages, paper presented at Trends in Chemical Dynamics: From Small Molecules to Biomolecules, Taiwan, R.O.C.: Academia Sinica, 2006.
  • Tsai, S.-T.; Liang, C.-W.; Chen, C.-H.; Wang, Y.-S., 2006, “Post-photoexcitation of gaseous biomolecules generated by laser desorption method”, P-52 pages, paper presented at Trends in Chemical Dynamics: From Small Molecules to Biomolecules, Taiwan, R.O.C.: Academia Sinica, 2006.
  • S.-T. Tsai, C.-W. Chen, L.-C. Huang, M.-C. Huang, C.-H. Chen and Y.-S. Wang, 2006, “Simultaneous mass analysis of positive and negative ions using a dual-polarity time-of-flight mass spectrometer”, Analytical Chemistry, 78 (22), 7729-7734.. (SCIE)
  • O. P. Charkin, N. M. Klimenko, D. O. Charkin, Y.-S. Wang, S.-C. Wei, H.-C. Chang and S. H. Lin, 2006, “Theoretical Study of the Structure and Stability of the Ferriporphyrin Dimer (Fe(III)C34H31N4O4)2”, RUSSIAN JOURNAL OF INORGANIC CHEMISTRY, 51 (1), 89. (SCIE)
  • Wang, Y.-S.; Wei, S.-C.; Chang, H.-C., 2005, “Photo-fragmentation of gaseous proteins in FT-ICR MS”, paper presented at The Fourth Asian Photochemistry Conference, Taiwan, R.O.C.: Academia Sinica, 2005-01-05 ~ 2005-01-12.
  • O. P. Charkin, N. M. Klimenko, T. P. Nguyen, D. O. Charkin, A. M. Mebel, S. H. Lin, Y.-S. Wang, S.-C. Wei and H.-C. Chang, 2005, “Fragmentation of heme and hemin(+) with sequential loss of carboxymethyl group: A DFT and mass-spectrometry study”, Chemical Physics Letters, 415 (4-6), 362-369. (SCIE)
  • O. P. Charkin, N. M. Klimenko, T. P. Nguyen, D. O. Charkin, Y.-S. Wang, S.-C. Wei, H.-C. Chang and S. H. Lin, 2005, “Theoretical study of fragmentation of heme and its ion with successive removal of carboxymethyl groups”, Russian Journal of Inorganic Chemistry, 50 (9), 1398-1407. (SCIE)
  • C. K. Lin, C. C. Wu, Y.-S. Wang, Y. T. Lee, H.-C. Chang, J. L. Kuo and M. L. Klein, 2005, “Vibrational predissociation spectra and hydrogen-bond topologies of H+ (H2O)(9-11)”, Physical Chemistry Chemical Physics, 7 (5), 938-944. (SCIE)
  • Wang, Y.-S.; Wei, S.-C.; Sahadevan, S.; Han, C.-C.; Chang, H.-C., 2004, “Collision-induced loss of charged versus neutral heme from gaseous myoglobin ions in an FTICR cell”, paper presented at The 52nd American Society for Mass Spectrometry conference on Mass Spectrometry and Allied Topics, Tennessee, U.S.A.: American Society for Mass Spectrometry, 2004.
  • C.-C. Wu, Y.-S. Wang, C. Chaudhuri, J.-C. Jiang and H.-C. Chang, 2004, “Microsolvation of the lithium ion by methanol in the gas phase”, Chemical Physics Letters, 388 (4-6), 457-462. (SCIE)
  • J. Friedrich, J. M. Fu, C. L. Hendrickson, A. G. Marshall and Y.-S. Wang, 2004, “Time resolved laser-induced fluorescence of electrosprayed ions confined in a linear quadrupole trap”, Review of Scientific Instruments, 75 (11), 4511-4515. (SCIE)
  • B. Cage, J. Friedrich, R. B. Little, Y.-S. Wang, M. A. McFarland, C. L. Hendrickson and A. G. Marshall, 2004, “Wavelength resolved laser-induced fluorescence emission of C6F3H3+ trapped in an ion cyclotron resonance cell”, Chemical Physics Letters, 394 (1-3), 188-193. (SCIE)
  • Y.-S. Wang, C.-H. Tsai, Y. T. Lee, H.-C. Chang, J. C. Jiang, O. Asvany, S. Schlemmer and D. Gerlich, 2003, “Investigations of protonated and deprotonated water clusters using a low-temperature 22-pole ion trap”, Journal of Physical Chemistry A, 107 (21), 4217-4225. (SCIE) (IF: 2.6; SCI ranking: 56%,40.5%)
  • Friedrich, J.; Cage, B.; Wang, Y.-S.; Little, R. B.; Hendrickson, C. L.; Marshall, A. G.,, 2003, “Wavelength Resolved Fluorescence Emission from Ions Trapped in an Ion Cyclotron Resonance (ICR) Cell”, paper presented at The 51st American Society for Mass Spectrometry conference on Mass Spectrometry and Allied Topics, Quebec, Canada: American Society for Mass Spectrometry, 2003.
  • Wang, Y.-S.; Asvany, O.; Tsai, C.-H.; Lee, Y.-T.; Chang, H.-C.; Schlemmer, S.; Gerlich, D., 2002, “Investigations of Protonated and Deprotonated Water Clusters Using a Temperature-Variable 22-Pole Ion Trap”, paper presented at Gordon Research Conferences, Program of Molecular & Ionic Clusters, California, U.S.A.: Gordon Research Conferences, 2002.
  • C. Chaudhuri, Y.-S. Wang, J. C. Jiang, Y. T. Lee, H.-C. Chang and G. Niedner-Schatteburg, 2001, “Infrared spectra and isomeric structures of hydroxide ion-water clusters OH- (H2O)(1-5): a comparison with H3O+ (H2O)(1-5)”, Molecular Physics, 99 (14), 1161-1173. (SCIE)
  • J. C. Jiang, Y.-S. Wang, H.-C. Chang, S. H. Lin, Y. T. Lee, G. Niedner-Schatteburg and H.-C. Chang, 2000, “Infrared spectra of H+(H2O)(5-8) clusters: Evidence for symmetric proton hydration”, Journal of the American Chemical Society, 122 (7), 1398-1410. (SCIE)
  • H.-C. Chang, J. C. Jiang, H.-C. Chang, Y.-S. Wang, S. H. Lin and Y. T. Lee, 1999, “On the search for H5O2+-centered water clusters in the gas phase”, Journal of the Chinese Chemical Society, 46 (3), 427-435. (SCIE)
  • Y.-S. Wang, H.-C. Chang, J. C. Jiang, S. H. Lin, Y. T. Lee and H.-C. Chang, 1998, “Structures and isomeric transitions of NH4+(H2O)(3-6): From single to double rings”, Journal of the American Chemical Society, 120 (34), 8777-8788. (SCIE)
  • H.-C. Chang, Y.-S. Wang, Y. T. Lee and H.-C. Chang, 1998, “Studying protonated ion hydration by infrared spectroscopy of size-selected NH4+(H2O)(n) clusters in a free jet expansion”, International Journal of Mass Spectrometry, 179/180, 91-102. (SCIE)
  • Y.-S. Wang, J. C. Jiang, C.-L. Cheng, S. H. Lin, Y. T. Lee and H.-C. Chang, 1997, “Identifying 2- and 3-coordinated H2O in protonated ion water clusters by vibrational pre-dissociation spectroscopy and ab initio calculations”, Journal of Chemical Physics, 107 (22), 9695-9698. (SCIE)