語言

Lu, Joyce Jean

JLu6x7Associate Professor

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+886-2-2787-1247
[ CV ]

 

 

EDUCATION AND POSITIONS HELD:

  • Associate Professor, Stem Cell Program, Genomics Research Center, Academia Sinica, Taiwan. 2015-present
  • Assistant Professor, Stem Cell Program, Genomics Research Center, Academia Sinica, Taiwan. 2007-2015
  • Adjunct Assistant Professor, Genomics and System Biology Program, College of Life Science, National Taiwan University, Taiwan. 2010-present
  • Co-PI, National RNAicore Facility Plateform, 2009-present
  • Postdoctoral Fellow/Associate, Molecular, Department of Cellular, and Developmental Biology, Yale University, USA. 2003-2007
  • Postdoctoral Fellow, Graduate Institute of Microbiology, College of Medicine, National Taiwan University, Taiwan. 2001-2003
  • Ph.D. Institute of Microbiology, National Taiwan University, Taipei, Taiwan. 1995-2000
  • M.S. Institute of Molecular Medicine, National Taiwan University, Taipei, Taiwan. 1992-1994
  • B.S. Department of Medical Technology, National Taiwan University, Taipei, Taiwan. 1988-1992
  • Research Assistant, Graduate Institute of Microbiology, College of Medicine, National Taiwan University, Taiwan. 1994-1995

HONORS:

  • Ruth L. Kirschstein National Research Service Award, 2003-2004, NIH
  • Outstanding Paper Award, National Taiwan University, 2002

RESEARCH INTERESTS:

High throughput functional screens pinpoints key factors for cell fate determination

Lu-scheme  Research Figure/Table/Scheme

Stem cell renewal and differentiation is important for stem cell biology and regenerative medicine. To efficiently pinpoint factors critical for cell fate determination, systematically functional screens were performed in mouse embryonic stem cells (ESCs), human ESCs, and mesenchymal stem cells (MSCs). We established the first shRNA screen in ESCs. With a shRNA library of kinases and phosphatases contains 4796 shRNAs, we identified 244 genes essential for ESC renewal. Among them, Nme6 and Nme7 is essential for 8 stemness genes expressions. With 517 shRNAs target genes are differentially expressed in undifferentiated human ESCs and differentiated cells or are embryonic lethal, we performed a shRNA functional screen and found 86 genes essential for human ESC renewal. And two candidate genes either bridge the oxidation and reduction status of ESCs with stemness signals, or block neural stem cell differentiation are currently studied in-depth. To fulfill the unmet need of bone diseases, a gain-of-function screen was performed with an overexpression library contains 12380 genes. 9 candidate genes are found to be essential and sufficient to promote the osteogenesis of human MSCs. By this method, we found soluble factor that can completely treat and prevent osteoporosis. Thus we found many factors play critical roles for ESC and MSC renewal and differentiation by systematically functional screens.

SELECTED PUBLICATIONS: