副研究員

EDUCATION AND POSITIONS HELD:

• Ph.D., Chemistry, University of Cambridge (UK), 2004
• Specialist, National Quarantine Service, Department of Health, Executive Yuan, Taipei, Taiwan, 1991.6-1991.12
• Specialist, Chemistry Division, National Laboratories of Foods & Drugs, Department of Health, Executive Yuan, Taipei, Taiwan, 1991-1995
• Teaching assistant, Food Science & Technology, The Ohio State University, 1995-1997
• Specialist, Microbiology Division, National Laboratories of Foods and Drugs, Department of Health, Executive Yuan, Taipei, Taiwan, 1998-2000
• Associate Research Scientist, University of Cambridge, 2003-2004
• Postdoctoral Fellow, Genomics Research Center, 2004-2004
• Assistant Professor, Biotechnology, Food Science, National Taiwan Ocean University, 2004-2007
• Assistant Research Fellow, Genomics Research Center, Academia Sinica, 2007-2011
• Adjunct Assistant/Associate Professor, Biotechnology Center, National Chung Hsing University, 2009-present
• Adjunct Assistant/Associate Professor, Marine Biotechnology Center, National Sun Yat-sen University, 2010-present
• Associate Research Fellow, Genomics Research Center, Academia Sinica, 2011-present 

HONORS:

• National Certificate, Professionals and Technologists in Nutrition, 1991
• National Certificate, National Senior Civil Service Examination (Environmental Hygiene), 1991
• EPA Certificates, Environmental Toxicology (EPA, Taiwan), 1994
• Annual Research Award (Department of Health), 1996
• National Annual Research Award (Executive Yuan), 1997
• National Certificate, National Advanced Senior Civil Service Examination (Hygiene Analysis), 1998
• Overseas PhD Fellowship, Ministry of Education (Medical Chemistry), 1998

RESEARCH INTERESTS:

Natural product chemistry

Microbial pathogenicity

The lab is also involved in a multidisciplinary study for characterizing mechanisms of microbial pathogenicity. The current targeted microbe is Klebsiella pneaumoniae K1, which is the major causative bacterium of hospital-acquired, debilitated and immuno-compromised infections in Taiwan. Its complete genome has been sequenced recently. The overall goal of the program project is to understand the relationship between virulence, CPS (capsular polysaccharide) structure, and biosynthesis of the CPS.

我們的研究工作主要著重於天然產物化學，特別是對於天然產物生合成路徑的闡釋及運用基因工程來發展更具生理效能的藥物。目前研究的重點天然產物包含：醣胜 肽類藥物(glycopeptides: mannopeptimycin, enduracidine, A40926, teicoplanin, chloroeremomycin), 氨基糖類藥物(aminoglycosides: moenomycin, minosaminomycin)及海洋毒素(marine toxins: tetrodotoxin)等。此外，我們也參與探討微生物致病性的群體研究，目前研究重點是針對一株對台灣公共衛生造成潛在威脅的克雷伯氏菌—K1 (Klebsiella Pneumoniae K1)，我們正進行此菌表面多醣體分子的成份與結構分析，及其獨特表面多醣體分子的生合成機制與侵入宿主所引發免疫反應等的研究，期以建構克雷伯氏菌的致病模型。

RESEARCH HIGHLIGHTS:

             Interception of teicoplanin oxidation intermediates yields new antimicrobial scaffolds

1. Overcoming resistance (The Scientist)
2. Dbv29 translation (SciBX) 
3. Building a better germ-killer (A-IMBN) 
4. 抗生素興亡史 (科學人第112期2011年6月號) 
5. 李宗璘博士研究團隊以調整酵素方式成功增強現有抗生素藥效 (中央研究院 院新聞稿) 
6. 巧手調整酵素，李宗璘實驗室以綠化學解除抗藥性窘境 (中央研究院基因體研究中心 新聞) 
7. 醣胜肽類抗生素生合成酵素之探討 (中央研究院 週報第1125期 知識天地) 
8. 功能基因體研究群發現關鍵酵素，可解決抗生素失效困境 (中央研究院基因體研究中心 新聞)

SELECTED PUBLICATIONS:

• H. Wang, Y. L. Chan, T. L. Li, B. A. Bauer, S. Hsia, C. H. Wang, J. S. Huang, H. M. Wang, K. Y. Yeh, T. H. Huang,C. J. Wu*, 2013, “Reduction of splenic immunosuppressive cells and enhancement of anti-tumor immunity by synergy of fish oil and selenium yeast”, PLOS ONE, 8(1):e52912. (SCI)
• T. L. Lin, F. L. Yang, A. S. Yang, H. P. Peng, T. L. Li, M. D. Tsai, S. H. Wu, J. T. Wang*, 2012, “Amino Acid Substitutions of MagA in Klebsiella pneumoniae Affect the Biosynthesis of the Capsular Polysaccharide”, PLOS ONE, 7(10), e46783. (SCI)
• H. C. Wu, Y. S. Li, Y. C. Liu, S. Y. Lyu, C. J. Wu, T. L. Li*, 2012, “Chain elongation and cyclization in type III PKS DpgA”, CHEMBIOCHEM, 13(6), 862-871. (SCI)
• T. L. Li*, Y. C. Liu, S. Y. Lyu, 2012, “Combining biocatalysis and chemoselective chemistries for glycopeptide antibiotics modification”, CURRENT OPINION IN CHEMICAL BIOLOGY, 16, 170-178. (SCI)
• S. H. Chen, D. R. Hwang, G. H. Chen, N. S. Hsu, Y. T. Wu, T. L. Li*, C. H. Wong*, 2012, “Engineering transaldolase in Pichia stipitis to improve bioethanol production”, ACS CHEMICAL BIOLOGY, 7(3), 481-486. (SCI)
• H. J. Chai, Y. L. Chan, T. L. Li, C. H. Wu, Y. C. Chen, C. Y. Shiau, C. J. Wu*, 2012, “Composition characterization of Myctophids (Benthosema pterotum): antioxidation and safety evaluations for Myctophids protein hydrolysates”, FOOD RESEARCH INTERNATIONAL, 46, 118-126. (SCI)
• H. Wang, Y. L. Chan, T. L. Li, C. J. Wu*, 2012, “Improving cachectic symptoms and immune strength of tumor-bearing mice in chemotherapy by a combination of Scutellaria baicalensis and Qing-Shu-Yi-Qi-Tang”, EUROPEAN JOURNAL OF CANCER, 48, 1074-1084. (SCI)
• Y. H. Chiu, Y. L. Chan, T. L. Li, C. J. Wu*, 2012, “Inhibition of Japanese encephalitis virus infection by the sulfated polysaccharide extracts from Ulva lactuca”, MARINE BIOTECHNOLOGY, 14, 468-478. (SCI)
• Y. H. Chiu, Y. L. Chan, L. W. Tsai, T. L. Li, C. J. Wu*, 2012, “Prevention of human enterovirus 71 infection by carrageenan”, ANTIVIRAL RESEARCH, 95, 128-134. (SCI)
• J. Y. Ho, T. L. Lin, A. Lee, A. N. Cheng, C. Y. Li, M. C. Chen, S. H. Wu, J. T. Wang*, T. L. Li*, M. D. Tsai*, 2011, “Functions of some capsular polysaccharide biosynthetic genes in Klebsiella pneumoniae NTUH K-2044”, PLOS ONE, 6(7), e21664. (SCI)
• H. Wang, Y. J. Lai, Y. L. Chan, T. L. Li, C. J. Wu*, 2011, “Epigallocatechin-3-gallate effectively attenuates skeletal muscle atrophy caused by cancer cachexia”, CANCER LETTERS, 305(1):40-49. (SCI)
• Y. C. Liu, Y. S. Li, S. Y. Lyu, L. J. Hsu, Y. H. Chen, Y. T. Huang, H. C. Chan, C. J. Huang, G. H. Chen, C. C. Chou, M. D. Tsai, T. L. Li*, 2011, “Interception of teicoplanin oxidation intermediates yields new antimicrobial scaffolds ”, NATURE CHEMICAL BIOLOGY, 7, 304-309. (SCI)
• C. J. Wu, H. Wang, Y. L. Chan, T. L. Li, 2011, “Passive immune-protection of small abalone against Vibrio alginolyticus infection by anti-Vibrio IgY-encapsulated feed”, FISH & SHELLFISH IMMUNOLOGY, 30, 1042-1048. (SCI)
• H. C. Chan, Y. T. Huang, S. Y. Lyu, C. J. Huang, Y. S. Li, Y. C. Liu, C. C. Chou, M. D. Tsai, and T. L. Li*, 2011, “Regioselective deacetylation based on teicoplanin-complexed Orf2* crystal structures”, MOLECULAR BIOSYSTEMS, 7(4), 1224-1231. (SCI)
• H. J. Chai, J. H. Li, H. N. Huang, T. L. Li, Y. L. Chan, C. Y. Shiau, C. J. Wu*, 2010, “Effects of sizes and conformations of fish scale collagen peptides (FSCPs) on facial skin qualities and transdermal penetration efficiency”, J BIOMED BIOTECHNOL, 2010, 757301. (SCI)
• Y. T. Huang, S. Y. Lyu, P. H. Chuang, N. S. Hsu, Y. S. Li, H. C. Chan, C. J. Huang, Y. C. Liu, C. J. Wu, W. B. Yang, T. L. Li*, 2009, “In vitro characterization of enzymes involved in the synthesis of nonproteinogenic residue (2S,3S)-b-methylphenylalanine in glycopeptide antibiotic mannopeptimycin”, CHEMBIOCHEM, 10, 2480-2487. (SCI)
• H. N. Huang, T. L. Li, Y. L. Chan, C. L. Chen, C. J. Wu*, 2009, “Transdermal immunization with low-pressure-gene-gun mediated chitosan-based DNA vaccines against Japanese encephalitis virus”, BIOMATERIALS, 30(30), 6017-6025. (SCI)
• J. Y. Cheng, H. N. Huang, W. C. Tseng, T. L. Li, Y. L. Chan, K. C. Cheng, C. J. Wu, 2009, “Transcutaneous immunization by lipoplex-patch based DNA vaccines is effective vaccination against Japanese encephalitis virus infection”, J CONTROL RELEASE, 135, 242-249. (SCI)
• T. L. Li*, D. Spiteller, J. B. Spencer, 2009, “Identification of a pentaketide stilbene produced by a type III polyketide synthase from Pinus sylvestris and characterisation of free coenzyme A intermediates”, CHEMBIOCHEM, 10, 896-901. (SCI)
• Y. S. Li, J. Y. Ho, C. C. Huang, S. Y. Lyu, C. Y. Lee, Y. T. Huang, C. J. Wu, H. C. Chan, C. J. Huang, N. S. Hsu, M. D. Tsai and T. L. Li*, 2007, “A unique flavin mononucleotide-linked primary alcohol oxidase for glycopeptide A40926 maturation”, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 129, 13384-13385. (SCI)
• C. J. Wu*, T. L. Li, H. W. Huang, Y. L. Chan and M. H. Tao, 2006, “Development of an effective Japanese encephalitis virus-specific DNA vaccine”, MICROBES AND INFECTION, 8, 2578-2586. (SCI)
• J. Y. Ho, Y. T. Huang, C. J. Wu, Y. S. Li, M. D. Tsai and T. L. Li*, 2006, “Glycopeptide Biosynthesis: Dbv21/Orf2* from dbv/tcp Gene Clusters Are N-Ac-Glm Teicoplanin Pseudoaglycone Deacetylases and Orf15 from cep Gene Cluster Is a Glc-1-P Thymidyltransferase”, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 128(42), 13694-13695. (SCI)
• F. Huang, S. F. Haydock, D. Spiteller, T. Mironenko, T. L. Li, P. F. Leadlay, D. O'Hagan and J. B. Spencer*, 2006, “The gene cluster for fluorometabolite biosynthesis in Streptomyces cattleya: a thioesterase confers resistance to fluoroacetyl-coenzyme A.”, CHEMISTRY & BIOLOGY, 13(5), 475-484. (SCI)
• T. L. Li, F. Huang, S. F. Haydock*, T. Mironenko, P. F. Leadlay and J. B. Spencer*, 2004, “Biosynthetic gene cluster of the glycopeptide antibiotic teicoplanin: characterization of two glycosyltransferases and the key acyltransferase”, CHEMISTRY & BIOLOGY, 11(1), 107-119. (SCI)
• T. L. Li*, Y. J. Chung-Wang and Y. C. Shih, 2002, “Determination and confirmation of chloramphenicol residues in swine muscle and liver”, JOURNAL OF FOOD SCIENCE, 67(1), 21-28. (SCI)
• T. L. Li, O. W. Choroba, H. Hong, D. H. Williams and J. B. Spencer*, 2001, “Biosynthesis of the vancomycin group of antibiotics: characterisation of a type III polyketide synthase in the pathway to (S)-3,5-dihydroxyphenylglycine”, CHEMICAL COMMUNICATIONS, 20, 2156-2157. (SCI)
• T. L. Li, O. W. Choroba, E. H. Charles, A. M. Sandercock, D. H. Williams and J. B. Spencer*, 2001, “Characterisation of a hydroxymandelate oxidase involved in the biosynthesis of two unusual amino acids occurring in the vancomycin group of antibiotics”, CHEMICAL COMMUNICATIONS, -18, 1752-1753. (SCI)
• T. L. Li, W. C. Lee*, P. C. Chang and S. S. Chou, 2001, “High performance chromatographic determination of asulam residue in agricultural products”, JOURNAL OF FOOD AND DRUG ANALYSIS, 9(1), 40-44. (SCI)
• W. C. Lee, T. L. Li*, P. C. Chang and S. S. Chou, 2001, “High performance liquid chromatographic determination of maleic hydrazide residue in potatoes”, JOURNAL OF FOOD AND DRUG ANALYSIS, 9(3), 167-172. (SCI)
• T. L. Li, J. M. King and Y. B. Min*, 2000, “Quenching mechanisms and kinetics of carotenoids in riboflavin photosensitized singlet oxygen oxidation of vitamin D-2”, JOURNAL OF FOOD BIOCHEMISTRY, 24(6), 477-492. (SCI)
• D. B. Min*, T. L. Li and H. O. Lee, 1998, “Effects of processing steps on the contents of minor compounds and oxidation of soybean oil”, ADV EXP MED BIOL, 434, 161-180. (SCI)
• T. L. Li* and D. B. Min*, 1998, “Stability and photochemistry of vitamin D-2 in model system”, JOURNAL OF FOOD SCIENCE, 63(3), 413-417. (SCI)
• W. C. Lee, T. L. Li*, W. J. Cheng, P. C. Chang and S. S. Chou, 1998, “Survey of nicotine and tar yields of domestic and imported cigarettes”, JOURNAL OF FOOD AND DRUG ANALYSIS, 6(4), 691-701. (SCI)