FACSVantageDiVa (Becton, Dickinson and Company)
FACSCalibure^TM (Becton, Dickinson and Company)
CyAn^TM LX Flow Cytometer S2504 (DakoCytomation)

Facility PI: Dr. Alice Yu
Lab tel :+886-2-2789-8750

FACSVantage

Our current FACSVantageDiVa cell sorting system consists of three lasers (an Innova® 305C Argon Ion laser, an Innova® 302C Krypton Ion laser and a Spectra Physics Helium-Neon laser) which generate three laser beams (a split beam of 488nm and 337.5-356.4 nm and 633 nm respectively). The system is equipped with ten detectors of which eight are for fluorescence detection and is capable of sorting on a maximum of eight color parameters in addition to the two standard forward and side scatter parameters. It is equipped with a TurboSort Plus module for high speed cell sorting (25,000 events/second) under multiple laser beam excitation and allows a maximum of 4-way non-rectangular sort windows.

FACSAria

FACSAria cell sorting system consists of three lasers (Sapphire, Cy355 and a Cube) which generate three laser beams (a split beam of 488nm and 355nm and 633 nm respectively). The system is equipped with twelve detectors of which ten are for fluorescence detection and is capable of sorting on a maximum of ten color parameters in addition to the two standard forward and side scatter parameters. It is for high speed cell sorting (70,000 events/second) under multiple laser beam excitation and allows a maximum of 4-way non-rectangular sort windows.

In addition the ACDU module allows single or multiple cell deposition onto microscopic slides and into individual wells of 60-, 96-, 24-well microtiter plates for subsequent user-defined analysis. Current

applications in GRC include multi-color parameters sorting of mouse hematopoietic stem cells, human

cord blood/marrow hematopoietic stem/progenitor cells and human leukemic stem cells, and sorting of cells based on gene expression followed by culture in vitro and/or transplantation in vivo.

FACSCalibur / FACSCanto

The BD FACSCalibureTM flow cytometer is a dual-laser four-color analyzer whereas the BD FACSCanto is a newer dual-laser six-color analyzer system. Both systems are user friendly and primarily for immunotyping and analysis of the level of protein expression when gene(s) of interest are ectopically expressed in target cells. Current applications in GRC are drug screening and monitor the effect of drugs on the development and functional activation of immune effecter cells.