GRC Mass Spectrometry Facility
Lab tel :+886-2-2789-8754
A hybrid linear ion trap quadrupole fourier-transform (LTQ-FT) mass spectrometer which combines fast mass analysis capability of linear ion trap with ultra high mass accuracy and ultra high mass resolution of 7.4 tesla FT mass spectrometer can provide sensitive proteomic analysis for complicated mixture with on-line nano scale liquid chromatography.
Beside conventional collision induced dissociation at LTQ, extra dissociation capabilities aiming for protein’s post-translation modifications (PTM) are realized by electron capture dissociation (ECD) and infrared multi-photon dissociation (IRMPD) at FT.
Linear ion trap quadrupole (LTQ XL) interfacing on-line LC, atmospheric matrix-assisted laser desorption/ionization (APMALDI) and micro scale chip-based nano electrospray ionization (Triversa Nanomate) can provide high order tandem mass analysis of phosphor and glyco proteomes with the aid of electron transfer dissociation (ETD).
Matrix assisted laser desorption/ionization time-of-flight (MALDI TOF) mass spectrometer (UltraFlex II) provides fast sequence determination of peptide and oligosaccharide.
The BioTOF III is robust for applications that require high resolving power and accurate mass measurement in proteomics, drug discovery, metabolomics, or organic chemistry. Its flexibility and wide mass range make it also ideal for the study of intact proteins or non-covalently bound complexes such as drug-protein, protein-protein, and protein-ligand interactions.
With a resolution of > 20,000 for a single pass mode and mass accuracy better than 4 ppm, the BioTOF III is the highest performance ESI-TOF commercially available. The built in coaxial multipass mode makes the resolution > 40,000.A GC-MS (Polaris Q) provides routine chemical identity verification with NIST chemical library for volatile chemicals.
- GRC Mass Spectrometry Facility
- GRC NMR Core Facility
- High Performance Liquid Chromatograph Facility
- Super High Throughput Drug Screening System
- Peptide Synthesis Core Facility
- Flow Cytometry Facility
- GRC Biosafety Level 3 Laboratory
- Protein X-ray Diffractometer
- Liquid Nitrogen CryoStorage
- Biochip Reader