Wu, Chung-Yi

Research Fellow

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Telephone: +886-2-27871263
[ CV ]




  • Ph D., Applied Chemistry, National Chiao-Tung University, Hsinchu, Taiwan, 2000
  • Postdoctoral Fellow, Academia Sinica, Taiwan, 2000-2006
  • Postdoctoral Fellow, The Scripps Research Institute, USA, 2004-2006
  • Assistant Research Fellow, Genomics Research Center, Academia Sinica, 2006-2011
  • Associate Research Fellow, Genomics Research Center, Academia Sinica, 2011-2016
  • Research Fellow, Genomics Research Center, Academia Sinica, 2016-present


  • Spring Prize of NCTU bronze medal, 1994
  • Postdoctoral Fellowship of Academia Sinica, 2000-2004
  • Cooperative Post-doctoral Research Visit Program of National Science Council, The Scripps Research Institute (USA), 2004-2006
  • Project for Excellent Junior Research Investigators Award, National Science Council, Taiwan, 2012
  • Career Development Award, Academia Sinica, Taiwan, 2012
  • Academia Sinica Research Award for Junior Research Investigators, 2012
  • The David Y. Gin New Investigator Award, American Chemical Society, 2014


Synthesis of oligosaccharide and development of sugar array.

More than 50% of human proteins are glycosylated. Glycomics has emerged with proteomics as an area for development and exploration in the postgenomic era. In comparison with genomics and proteomics, there are two major problems to defer the development of this field. The first is non automated synthesis of oligosaccharides platform exit. Second is needed to develop a more rapid method in glycoprotein sequencing, developing analytical and biochemical tools and biological readouts to investigate glycan structure-function relationships.
We focus on the development of novel and efficient methodologies to prepare biologically potent oligosaccharides and design the new type sugar array to address the bio-function of oligosaccharides.

Sugar array_and_its_applications Carbohydrate-based vaccines_design Ultrasensitive naked_eye_detection
      Glycan Array and Its Applications      Carbohydrate-based vaccines design        Ultrasensitive naked eye detection


  • Sachin S Shivatare, Shih-Huang Chang, Tsung-I Tsai, Susan Yu Tseng, Yih-Shyan Lin,Yang-Yu Cheng, Chien-Tai Ren, Chang-Chun David Lee, Sujeet Pawar, Charng-Sheng Tsai,Hao-Wei Shih, Yi-Fang Zeng, Chi-Hui Liang Peter D. Kwong, Dennis R. Burton, Chung-Yi Wu,* Chi-Huey Wong*, 2016, “Modular synthesis of N-glycans and arrays for the hetero-ligand binding analysis of HIV antibodies”, Nature Chemistry, 8(4), 338-346. (SCI)
  • Guillaume B. E. Stewart-Jones, Cinque Soto, Thomas Lemmin, Gwo-Yu Chuang, Aliaksandr Druz, Rui Kong, Paul V. Thomas, Kshitij Wagh, Tongqing Zhou, Anna-Janina Behrens, Tatsiana Bylund, Chang W. Choi, Jack R. Davison, Ivelin S. Georgiev, M. Gordon Joyce, Young Do Kwon, Marie Pancera, Justin Taft, Yongping Yang, Baoshan Zhang, Sachin S. Shivatare, Vidya S. Shivatare, Chang-Chun D. Lee, Chung-Yi Wu, Carole A. Bewley, Dennis R. Burton,, Wayne C. Koff, Mark Connors, Max Crispin, Ulrich Baxa, Bette T. Korber, Chi-Huey Wong, John R. Mascola and Peter D. Kwong*, 2016, “Trimeric HIV-1-Env Structures Define Glycan Shields from Clades A, B, and G.”, CELL, 165(4), 813-826. (SCI)
  • CW Lin, MH Tsai, ST Li, TI Tsai, KC Chu, YC Liu, MY Lai, YC Tseng, CY Wu, SS Shivatare, CH Wang, P Chao, SY Wang, HW Shih, YF Zeng, TH You, JY Liao, CL Chen, CS Tsai, CC Huang, NH Lin, C Ma, CY Wu*, CH Wong*, 2015, “A common glycan structure on immunoglobulin G for enhancement of effector functions.”, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 112(34), 10611-10616. (SCI)
  • Lee, H.-Y., Chen, C.-Y., Tsai, T.-I., Li, S.-T., Lin, K.-H., Cheng, Y.-Y., Ren, C.-T., Cheng, T.-J., Wu, C.-Y.*, Wong, C.-H.* , 2014, “Immunogenicity study of Globo H derivatives with modification at the reducing or non-reducing end of the tumor antigen”, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 136, 16844-16853. (SCI)
  • Chen-Shiou Wu, Chia-Jui Yen, Ruey-Hwang Chou, Chung-Yi Wu*, Yung-Luen Yu*, 2014, “Downregulation of MicroRNA-15b by Hepatitis B Virus X Enhances Hepatocellular Carcinoma Proliferation via Fucosyltransferase 2-Induced Globo H Expression”, INTERNATIONAL JOURNAL OF CANCER, 134(7), 1638-1647. (SCI)
  • Juine-Ruey Che, 2014, “Vaccination of Monoglycosylated Hemagglutinin Induces Cross-Strain Protection against Influenza Virus Infections”, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 111(7), 2476-2481. (SCI)
  • Sachin S Shivatare, Shih-Huang Chang, Tsung-I Tsai, Chein-Tai Ren, Hong-Yang Chuang, Li Hsu, Chih-Wei Lin, Shiou-Ting Li, Chung-Yi Wu,* and Chi-Huey Wong*, 2013, “Efficient Convergent Synthesis of Bi-, Tri-, and Tetraantennary Complex Type N-Glycans and Their HIV-1 Antigenicity.”, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 135(41), 15382-15391. (SCI)
  • Tsung-I Tsai, Hsin-Yu Lee, Shih-Huang Chang, Chia-Hung Wang, Yu-Chen Tu, Yu-Chen Lin, Der-Ren Hwang, Chung-Yi Wu, Chi-Huey Wong*, 2013, “Effective Sugar Nucleotide Regenertion for the Large-scale Enzymatic Synthesis of Globo H and SSEA4”, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 135(39), 14831-14839. (SCI)
  • S.-F Liao, C.-H. Liang, M.-Y. Ho, T.-L. Hsu, T.-I. Tsai, Y.S.-Y. Hsieh, S.-T. Li, Y.-Y. Cheng, S.-M. Tsao, T.-Y. Lin, Z.-Y. Lin, W.-B. Yang, C.-T. Ren, K.-I. Lin, K.-H. Khoo, C.-H. Lin, H.-Y. Hsu*, C.-Y. Wu*, C.-H. Wong*, 2013, “Immunization of fucosucosucose-containing polysaccharides from Reishi mushroom induced antibodies against tumor-associated Globo H-series epitopes.”, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 110(34), 13809-13814. (SCI)
  • Hong-Yang Chuang, Chien-Tai Ren, Chung-An Chao, Chung-Yi Wu, Sachin S Shivatare, Ting-Jen R. Cheng,Chung-Yi Wu*, and Chi-Huey Wong*, 2013, “Synthesis and Vaccine Evaluation of the Tumor Associated Carbohydrate Antigen RM2 from Prostate Cancer”, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 135(30), 11140-11150. (SCI)
  • Chia-Hung Wang, Shiou-Ting Li, Tzu-Lung Lin, Yang-Yu Cheng, Tsung-Hsien Sun, Jin-Town Wang, Ting-Jen R. Cheng, Kwok Kong Tony Mong, Chi-Huey Wong and Chung-Yi Wu*, 2013, “Synthesis of Neisseria meningitidis Serogroup W135 Capsular Oligosaccharides for Immunogenicity Comparison and Vaccine Development”, ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 52(35), 9157-9161. (SCI)
  • S.-C. Lin, J.-T. Jan, B. Dionne, M. Butler, M.-H. Huang, C.-Y. Wu, C.-H. Wong, S.-C. Wu, 2013, “ Different Immunity Elicited by Recombinant H5N1 Hemagglutinin Proteins Containing Pauci-mannose, High-mannose, or Complex type N-Glycans.”, PLoS One, 8(6), e66719. (SCI)
  • T.-C. Hung, C.-W. Lin, T.-L. Hsu, C.-Y. Wu, C.-H. Wong* , 2013, “Investigation of SSEA-4 Binding Protein in Breast Cancer Cells”, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 135(16), 5934-5937. (SCI)
  • Y.-L. Huang, J.-T. Hung, S. K.C. Cheung, H.-Y. Lee, K.-C. Chu, S.-T. Li, Y.-C. Lin, C.-T. Ren, T.-J. R. Cheng, T.-L. Hsu, A. L. Yu, C.-Y. Wu*, C.-H. Wong*, 2013, “Carbohydrate-based vaccines with a glycolipid adjuvant for breast cancer”, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 110(7), 2517-22. (SCI)
  • T.-J. R. Cheng, S.-Y. Wang, W.-H. Wen, C.-Y. Su, M. Lin, W.-I. Huang, M.-T. Liu, H.-S. Wu, N-S. Wang, C.-K. Cheng, C.-L. Chen, C.-T. Ren, C.-Y. Wu, J.-M. Fang,* Y.-S. E. Cheng* and C.-H. Wong*, 2013, “Chemical probes for drug resistance assessment by binding competition (RABC) oseltamivir susceptibility evaluation”, ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 52(1), 366-370. (SCI)
  • Wu C.-H., Yen C.-J., Chou R.-H., Li S.-T., Huang W.-C., Ren C.-T., Wu C.-Y.*, Yu Y.-L.*, 2012, “Cancer-Associated Carbohydrate Antigens as Potential Biomarkers for Hepatocellular Carcinoma.”, PLoS One, 7, e39466. (SCI)
  • Che-Hsiung Hsu, Shang-Cheng Hung*, Chung-Yi Wu*, and Chi-Huey Wong*, 2011, “Toward Automated Oligosaccharide Synthesis”, ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 50(50), 11872-11923. (SCI)
  • K.-C. Chu, C.-T. Ren, C.-P. Lu, C.-H. Hsu, T.-H. Sun, J.-L. Han, B. Pal, T.-A. Chao, S.-H. Wu, C.-H. Wong* and C.-Y. Wu*, 2011, “Efficient and Stereoselective Synthesis of alpha(2→9) Oligosialic Acids: From Monomers to Dodecamers”, ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 50, 9391-9395. (SCI)
  • H-L Yen, C-H Liang, C-Y Wu, H Forrest, A Ferguson, KT Choy, J Jones, DDY Wong, PPH Cheung, C-H Hsu, OT Li, KM Yuen, RWY Chan, LLM Poon, MCW Chan, J Nicholls, S Krauss, C-H Wong, Y Guan, RG Webster, RJ Webby & JSM Peiris* , 2011, “Hemagglutinin-neuraminidase balance confers respiratory droplet transmissibility of the pandemic H1N1 influenza virus in ferrets”, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 108, 14264-14269. (SCI)
  • Chung-Yi Wu* and Chi-Huey Wong*, 2011, “Chemistry and Glycobiology”, CHEMICAL COMMUNICATIONS, 47, 6201-6207. (SCI)
  • Liang, C.-H., Wang, S.-K., Lin, C.-W., Wong, C.-H.*, Wu, C.-Y.*, 2011, “Effects of neighboring glycans on antibody-carbohydrate interaction”, Angewandte chemie-international edition, 50(7), 1608-1612. (SCI)
  • Y.-L. Huang, C.-Y. Wu*, 2010, “Carbohydrate-Based Vaccines: Challenges and Opportunities”, Expert review of vaccines, 9 (11), 1257-1274. (SCI)
  • Hsu, C.-H.; Chu, K.-C.; Lin, Y.-S.; Han, J.-L.; Peng, Y.-S.; Ren, C.-T.; Wu, C.-Y.* and Wong, C.-H.*, 2010, “Highly Alpha-Selective Sialyl Phosphate Donors for Efficient Preparation of Natural Sialosides.”, Chemistry-A European Journal, 16(6), 1754-1760. (SCI)
  • Liao H-Y, Hsu C-H, Wang S-C, Liang C-H, Ye H-Y, Su C-Y, Jan J-T, Ren C-T, Cheng T.-J R,* Wu C-Y*, Wong C-H*, 2010, “Differential Receptor Binding Affinities of Influenza Hemagglutinins on Glycan Arrays”, Journal of the American Chemical Society, 132, 14849-14856. (SCI)
  • Shih-Huang Chang, Jeng-Liang Han, Susan Y. Tseng, Hsin-Yu Lee, Chin-Wei Lin, Yu-Chen Lin, Wen-Yih Jeng, Andrew H.-J. Wang, Chung-Yi Wu,* Chi-Huey Wong*, 2010, “Glycan Array on Aluminum Oxide Coated Glass Slides through Phosphonate Chemistry”, Journal of the American Chemical Society, 132, 13371-13380. (SCI)
  • Liang C-H, Wu,C-Y*, 2009, “Glycan Array: A Powerful Tool for Glycomics Studies”, Expert Review of Proteomics, 6(6), 631-645. (SCI)
  • Chi-Hui Linag,Cheng-Chi Wang,Yu-Chen Lin,Chung-Hsuan Chen,Chi-Huey Wong*,Chung-Yi Wu*, 2009, “Iron Oxide/Gold Core/Shell Nanoparticle for Ultrasensitive Detection of Carbohydrate-Protein Interactions”, Analytical Chemistry, 81, 7750-7756. (SCI)
  • Susan Y. Tseng, Cheng-Chi Wang, Chin-Wei Lin, Cheng-Lung Chen, Wen-Yueh Yu, Chung-Hsuan Chen, Chung-Yi Wu*, Chi-Huey Wong*, 2008, “Glycan arrays on aluminum-coated glass slides.”, CHEMISTRY-AN ASIAN JOURNAL, 3(8-9), 1395-1405.. (SCI)
  • Pi-Hui Liang, Chung-Yi Wu, William A. Greenberg, Chi-Huey Wong*, 2008, “Glycan arrays: biological and medical applications.”, CURRENT OPINION IN CHEMICAL BIOLOGY, 12(1), 86-92 2008. (SCI)
  • Cheng-Chi Wang,Yen-Lin Huang, Chien-Tai Ren, Chin-Wei Lin, Jung-Tung Hung, Jyh-Cherng Yu, Alice L. Yu*, Chung-Yi Wu*, Chi-Huey Wong*, 2008, “Glycan microarray of Globo H and related structures for quantitative analysis of breast cancer. ”, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 105(33), 11661-11666. (SCI)
  • J.C. Lee, Chung-Yi Wu, J.V. Apon, G. Siuzdak and C.H. Wong, 2006, “Reactivity-based one-pot synthesis of the tumor-associated antigen N3 minor octasaccharide for the development of a photocleavable DIOS-MS sugar array”, ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 45, 2753-2757. (SCI)
  • Chung-Yi Wu, K. Y. King, C. J. Kuo, J. M. Fang, Y. T. Wu, M. Y. Ho, C. L. Liao, J. J. Shie, P. H. Liang and C. H. Wong, 2006, “Stable Benzotriazole Esters as Mechanism-Based Inactivators of the Severe Acute Respiratory Stndrome 3CL Protease”, Chem Biol, 13, 261-268. (SCI)
  • Chung-Yi Wu, J. T. Jan*, S. H. Ma, C. J. Kuo, H. F. Juan, Y. H. E. Cheng, H. H. Hsu, R. H. Liu, J. M. Fang, P. H. Liang and C. H. Wong*, 2004, “Small molecules targeting Severe Acute Respiratory Syndrome (SARS) human coronavirus”, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 101, 10012-10017. (SCI)
  • Chung-Yi Wu, C. F. Chang, J. S. Y. Chen, C. H. Wong*, C. H. Lin*, 2003, “Rapid Diversity-Oriented Synthesis in Microtiter Plates for in situ Screening: Discovery of Potent and Selective a-Fucosidase Inhibitors”, ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 42(38), 4661-4664. (SCI)